Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) yeeUV (cbtA-cbeA)/CbtA-CbeA
Location 3724921..3725756 Replicon chromosome
Accession NZ_CP038302
Organism Escherichia coli O157:H7 strain SS TX 313-1

Toxin (Protein)


Gene name yeeV Uniprot ID A0A2A3UQ69
Locus tag E4U66_RS19230 Protein ID WP_000854714.1
Coordinates 3724921..3725298 (-) Length 126 a.a.

Antitoxin (Protein)


Gene name yeeU Uniprot ID A0A2A3UQI5
Locus tag E4U66_RS19235 Protein ID WP_001285574.1
Coordinates 3725388..3725756 (-) Length 123 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U66_RS19190 3720245..3720949 + 705 WP_001241680.1 leucyl/phenylalanyl-tRNA--protein transferase -
E4U66_RS19195 3721234..3721452 + 219 WP_001040187.1 translation initiation factor IF-1 -
E4U66_RS19205 3721943..3722224 - 282 Protein_3762 DUF4942 domain-containing protein -
E4U66_RS19210 3722506..3723510 - 1005 WP_001179707.1 IS110-like element ISSfl8 family transposase -
E4U66_RS19215 3723579..3724142 - 564 WP_001280462.1 DUF4942 domain-containing protein -
E4U66_RS19220 3724227..3724424 - 198 WP_000839249.1 DUF957 domain-containing protein -
E4U66_RS19225 3724436..3724924 - 489 WP_000761724.1 hypothetical protein -
E4U66_RS19230 3724921..3725298 - 378 WP_000854714.1 TA system toxin CbtA family protein Toxin
E4U66_RS19235 3725388..3725756 - 369 WP_001285574.1 type IV toxin-antitoxin system toxin CbtA Antitoxin
E4U66_RS19240 3725836..3726057 - 222 WP_000692328.1 DUF987 domain-containing protein -
E4U66_RS19245 3726120..3726596 - 477 WP_001186726.1 RadC family protein -
E4U66_RS19250 3726612..3727085 - 474 WP_001456342.1 antirestriction protein -
E4U66_RS19255 3727179..3727424 - 246 WP_000680582.1 hypothetical protein -
E4U66_RS19260 3727424..3728242 - 819 WP_001234742.1 DUF945 domain-containing protein -
E4U66_RS19265 3728332..3728555 - 224 Protein_3774 DUF905 family protein -
E4U66_RS19270 3728653..3729066 - 414 WP_000789534.1 hypothetical protein -
E4U66_RS19275 3729336..3729875 - 540 WP_001104024.1 DUF4339 domain-containing protein -
E4U66_RS19280 3730001..3730458 - 458 Protein_3777 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 3722506..3723510 1004


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

CbtA toxin of type IV toxin-antitoxin system

(1-112)

Antitoxin

Antitoxin CbeA

(18-117)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 14216.31 Da        Isoelectric Point: 7.8046

>T121238 WP_000854714.1 NZ_CP038302:c3725298-3724921 [Escherichia coli O157:H7]
MKTLPDTHVREASRCPSPVIIWQILLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVDNITLGKHPEAKR

Download         Length: 378 bp

>T121238 NZ_CP038302:c3725298-3724921 [Escherichia coli O157:H7]
ATGAAAACATTACCCGATACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCATCATCTGGCAGATTCTGCTCAC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAGATAACATTACCCTGGGGAAGCATCCGGAGGCGAAACGATGA

Antitoxin


Download         Length: 123 a.a.        Molecular weight: 13578.33 Da        Isoelectric Point: 6.3152

>AT121238 WP_001285574.1 NZ_CP038302:c3725756-3725388 [Escherichia coli O157:H7]
VSDTLPGTTHPDDNNDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPTPETKK

Download         Length: 369 bp

>AT121238 NZ_CP038302:c3725756-3725388 [Escherichia coli O157:H7]
GTGTCAGACACTCTCCCCGGGACAACGCATCCCGACGATAACAACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCCGGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTATATGCTAAAGGGCTGACCTGCGATGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACCCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A3UQ69

Antitoxin

Source ID Structure
AlphaFold DB A0A2A3UQI5

References