Detailed information of TA system TA121230

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2565676..2566147
• Replicon: chromosome
• Accession: NZ_CP038302
• Organism: Escherichia coli O157:H7 strain SS TX 313-1

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E4U66_RS12925
• Protein ID: WP_001303511.1
• Coordinates: 2565676..2565954 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E4U66_RS12930
• Protein ID: WP_001302048.1
• Coordinates: 2565956..2566147 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U66_RS12885	2560682..2560866	-	185	Protein_2517	DUF3596 domain-containing protein	-
E4U66_RS12890	2560901..2561152	-	252	WP_000005552.1	excisionase family protein	-
E4U66_RS12895	2561225..2563696	-	2472	WP_000048458.1	exonuclease	-
E4U66_RS12900	2563789..2563980	-	192	WP_001090200.1	DUF1482 family protein	-
E4U66_RS12905	2563977..2564165	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E4U66_RS12910	2564566..2564730	+	165	WP_001331716.1	hypothetical protein	-
E4U66_RS12915	2564734..2564952	-	219	WP_001171930.1	hypothetical protein	-
E4U66_RS12920	2565024..2565323	-	300	WP_001240334.1	hypothetical protein	-
E4U66_RS12925	2565676..2565954	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U66_RS12930	2565956..2566147	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E4U66_RS12935	2566168..2566539	-	372	WP_001169686.1	hypothetical protein	-
E4U66_RS12940	2566637..2566939	+	303	WP_000172738.1	transcriptional regulator	-
E4U66_RS12945	2566936..2567361	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E4U66_RS12950	2567384..2568346	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
E4U66_RS12955	2568353..2569093	+	741	WP_000788936.1	ATP-binding protein	-
E4U66_RS12960	2569119..2569888	+	770	Protein_2532	DUF1627 domain-containing protein	-
E4U66_RS12965	2569904..2570299	+	396	WP_001118159.1	DUF977 family protein	-
E4U66_RS12970	2570356..2570940	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2557766..2604815	47049

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T121230 WP_001303511.1 NZ_CP038302:c2565954-2565676 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T121230 NZ_CP038302:c2565954-2565676 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT121230 WP_001302048.1 NZ_CP038302:c2566147-2565956 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT121230 NZ_CP038302:c2566147-2565956 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0