Detailed information of TA system TA121202

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3572005..3572483
• Replicon: chromosome
• Accession: NZ_CP038300
• Organism: Escherichia coli O157:H7 strain SS TX 754-1

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: E4U76_RS18410
• Protein ID: WP_001303876.1
• Coordinates: 3572196..3572483 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: E4U76_RS18405
• Protein ID: WP_000536233.1
• Coordinates: 3572005..3572196 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U76_RS18370	3567409..3568179	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U76_RS18375	3568201..3568947	-	747	WP_000788751.1	ATP-binding protein	-
E4U76_RS18380	3568954..3570045	-	1092	WP_001205823.1	hypothetical protein	-
E4U76_RS18385	3570124..3570579	+	456	WP_000273724.1	hypothetical protein	-
E4U76_RS18390	3570786..3571211	-	426	WP_000693855.1	Rha family transcriptional regulator	-
E4U76_RS18395	3571195..3571467	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
E4U76_RS18400	3571576..3571977	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
E4U76_RS18405	3572005..3572196	+	192	WP_000536233.1	hypothetical protein	Antitoxin
E4U76_RS18410	3572196..3572483	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U76_RS18415	3572761..3572916	+	156	WP_000379575.1	DUF1391 family protein	-
E4U76_RS18420	3573058..3573447	+	390	WP_000394511.1	hypothetical protein	-
E4U76_RS18425	3573634..3573819	-	186	WP_001133046.1	hypothetical protein	-
E4U76_RS18430	3574393..3574581	+	189	WP_000413705.1	cell division inhibition protein DicB	-
E4U76_RS18435	3574578..3574769	+	192	WP_001098307.1	DUF1482 family protein	-
E4U76_RS18440	3574863..3577334	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3529201..3579708	50507

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T121202 WP_001303876.1 NZ_CP038300:3572196-3572483 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T121202 NZ_CP038300:3572196-3572483 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT121202 WP_000536233.1 NZ_CP038300:3572005-3572196 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT121202 NZ_CP038300:3572005-3572196 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA