Detailed information of TA system TA121178

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): parDE/RHH(antitoxin)
• Location: 35541..35971
• Replicon: plasmid pTB182A-5
• Accession: NZ_CP038299
• Organism: Escherichia coli O157:H7 strain TB182A

Toxin (Protein)

• Gene name: parE
• Uniprot ID: -
• Locus tag: E3171_RS25830
• Protein ID: WP_001388374.1
• Coordinates: 35541..35705 (-)
• Length: 55 a.a.

Antitoxin (Protein)

• Gene name: parD
• Uniprot ID: A0A6Y0EDY2
• Locus tag: E3171_RS25835
• Protein ID: WP_000079939.1
• Coordinates: 35702..35971 (-)
• Length: 90 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3171_RS25800	30958..31662	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
E3171_RS25805	31758..32267	+	510	WP_000571065.1	trimethoprim-resistant dihydrofolate reductase DfrA8	-
E3171_RS25810	32264..32920	+	657	WP_000144779.1	metallophosphoesterase	-
E3171_RS25815	32957..33661	+	705	WP_001067855.1	IS6-like element IS26 family transposase	-
E3171_RS25820	33748..34128	-	381	WP_001696636.1	HTH domain-containing protein	-
E3171_RS25825	34570..35493	-	924	WP_001415483.1	hypothetical protein	-
E3171_RS25830	35541..35705	-	165	WP_001388374.1	hypothetical protein	Toxin
E3171_RS25835	35702..35971	-	270	WP_000079939.1	hypothetical protein	Antitoxin
E3171_RS25840	36044..36184	-	141	WP_001404367.1	hypothetical protein	-
E3171_RS25845	36898..37920	-	1023	WP_032268691.1	replication initiation protein	-
E3171_RS25850	37913..37987	-	75	WP_071703955.1	RepA leader peptide Tap	-
E3171_RS25855	38223..38480	-	258	WP_047648528.1	replication regulatory protein RepA	-
E3171_RS25860	39037..39267	-	231	WP_170867393.1	hypothetical protein	-
E3171_RS25865	39600..40613	-	1014	WP_001404372.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(6)-Id / aph(3'')-Ib / sul2 / blaTEM-1B / dfrA8	-	1..70894	70894

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 55 a.a.
• Molecular weight: 6604.73 Da
• Isoelectric Point: 7.1989

>T121178 WP_001388374.1 NZ_CP038299:c35705-35541 [Escherichia coli O157:H7]
MMEIFWTMLASQDRKRIREYIAEQNLIAAIELDERIGYFGRTHRLTPVLHLYYM

Nucleotide

• Length: 165 bp

>T121178 NZ_CP038299:c35705-35541 [Escherichia coli O157:H7]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAATACATTGCGGAGCAGAACCTGAT
AGCAGCCATTGAACTGGACGAACGGATTGGTTATTTCGGACGAACGCACCGGCTTACGCCGGTTTTACATCTGTACTACA
TGTAA

Antitoxin

Protein

• Length: 90 a.a.
• Molecular weight: 10233.54 Da
• Isoelectric Point: 6.7263

>AT121178 WP_000079939.1 NZ_CP038299:c35971-35702 [Escherichia coli O157:H7]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLEHGDVLPHDMVESSAAAWRD
EMSRKVAGK

Nucleotide

• Length: 270 bp

>AT121178 NZ_CP038299:c35971-35702 [Escherichia coli O157:H7]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGTCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGAGCACGGCGATGTACTTCCCCATGACATGGTCGAATCCTCTGCTGCAGCATGGCGAGAT
GAAATGAGCAGGAAGGTTGCCGGTAAATGA

Structures

Antitoxin

• AlphaFold DB: A0A6Y0EDY2