Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) parDE/RelE-RHH
Location 49608..50155 Replicon plasmid pTB182A-4
Accession NZ_CP038298
Organism Escherichia coli O157:H7 strain TB182A

Toxin (Protein)


Gene name parE Uniprot ID A0A896ZGX1
Locus tag E3171_RS25490 Protein ID WP_000969997.1
Coordinates 49874..50155 (+) Length 94 a.a.

Antitoxin (Protein)


Gene name parD Uniprot ID A0A7U8WKC6
Locus tag E3171_RS25485 Protein ID WP_000079946.1
Coordinates 49608..49877 (+) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3171_RS25445 45310..45825 + 516 WP_000998852.1 type II secretion system protein M -
E3171_RS25450 45877..46710 + 834 WP_000088803.1 A24 family peptidase -
E3171_RS25455 46804..47205 + 402 WP_001222321.1 GspS family T2SS pilot lipoprotein variant EptO -
E3171_RS25460 47280..47495 + 216 Protein_64 IS3 family transposase -
E3171_RS25465 47794..48087 + 294 Protein_65 hypothetical protein -
E3171_RS25470 48316..48564 + 249 WP_000083841.1 replication regulatory protein RepA -
E3171_RS25475 48587..48694 + 108 Protein_67 incFII family plasmid replication initiator RepA -
E3171_RS25480 49395..49553 + 159 WP_000829588.1 hypothetical protein -
E3171_RS25485 49608..49877 + 270 WP_000079946.1 hypothetical protein Antitoxin
E3171_RS25490 49874..50155 + 282 WP_000969997.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
E3171_RS25495 50351..50692 - 342 WP_000578011.1 hypothetical protein -
E3171_RS25500 50992..51579 + 588 WP_001418189.1 serine acetyltransferase -
E3171_RS25505 51861..52454 - 594 WP_000517696.1 hypothetical protein -
E3171_RS25510 52808..54154 + 1347 WP_001132032.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid - nleA/espI / stcE / exeE / exeG 1..66022 66022


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(4-92)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 94 a.a.        Molecular weight: 10879.54 Da        Isoelectric Point: 8.4615

>T121177 WP_000969997.1 NZ_CP038298:49874-50155 [Escherichia coli O157:H7]
MMEIFWTMLASQDRKRIREYIAEQNLMAAIELDERIGYSASSLAGQPYKGRNGRVEGTRELVIHPHFVLVYEVDSQWGKV
YVLRVLHTAQKWP

Download         Length: 282 bp

>T121177 NZ_CP038298:49874-50155 [Escherichia coli O157:H7]
ATGATGGAGATATTCTGGACCATGCTGGCCAGCCAGGACAGGAAGCGCATTCGTGAGTACATTGCGGAGCAGAACCTGAT
GGCCGCCATTGAACTGGACGAACGGATTGGTTATTCAGCCAGCAGCCTGGCCGGGCAGCCGTATAAAGGTCGCAACGGTC
GTGTGGAAGGAACCCGGGAACTGGTTATTCACCCCCATTTTGTTCTGGTTTATGAGGTCGACAGCCAGTGGGGAAAAGTG
TATGTCCTGCGCGTGTTGCATACCGCACAGAAGTGGCCATAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 10210.55 Da        Isoelectric Point: 8.8735

>AT121177 WP_000079946.1 NZ_CP038298:49608-49877 [Escherichia coli O157:H7]
MAQVNMSLRIDAELKDAFMAAAKSMDRNGSQLIRDFMRQTVERQHNTWFRDQVEAGRQQLGRGDVLPHDMVESSATAWRD
EMSRKVAGK

Download         Length: 270 bp

>AT121177 NZ_CP038298:49608-49877 [Escherichia coli O157:H7]
ATGGCACAGGTTAATATGAGTTTAAGAATCGACGCTGAACTGAAGGATGCTTTTATGGCCGCTGCAAAAAGCATGGACCG
TAATGGCTCTCAGTTAATCCGGGATTTTATGCGCCAGACCGTTGAACGGCAGCATAATACCTGGTTCCGTGACCAGGTTG
AGGCAGGACGTCAGCAACTCGGGCGTGGTGATGTGCTTCCACATGACATGGTCGAATCCTCCGCGACTGCATGGCGGGAT
GAAATGAGCAGAAAGGTCGCCGGTAAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A896ZGX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U8WKC6

References