Detailed information of TA system TA121176

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/ParE-YafN
• Location: 10513..11048
• Replicon: plasmid pTB182A-4
• Accession: NZ_CP038298
• Organism: Escherichia coli O157:H7 strain TB182A

Toxin (Protein)

• Gene name: relE
• Uniprot ID: -
• Locus tag: E3171_RS25205
• Protein ID: WP_001270421.1
• Coordinates: 10513..10800 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: A0A1V0M724
• Locus tag: E3171_RS25210
• Protein ID: WP_001132893.1
• Coordinates: 10797..11048 (-)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3171_RS25170	5916..6095	-	180	WP_000526857.1	hypothetical protein	-
E3171_RS25175	6298..6672	+	375	WP_001368887.1	hypothetical protein	-
E3171_RS25180	6983..7144	-	162	Protein_8	epoxyqueuosine reductase QueH	-
E3171_RS25185	7195..7335	+	141	Protein_9	serine/threonine protein kinase	-
E3171_RS25190	7459..8199	+	741	WP_001066926.1	tyrosine-type recombinase/integrase	-
E3171_RS25195	8483..9460	-	978	WP_000361616.1	RepB family plasmid replication initiator protein	-
E3171_RS25200	10106..10511	-	406	Protein_12	DDE-type integrase/transposase/recombinase	-
E3171_RS25205	10513..10800	-	288	WP_001270421.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E3171_RS25210	10797..11048	-	252	WP_001132893.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3171_RS25215	11437..12642	+	1206	WP_000817637.1	AAA family ATPase	-
E3171_RS25220	12639..13601	+	963	WP_000756329.1	ParB/RepB/Spo0J family partition protein	-
E3171_RS25225	13742..14753	-	1012	Protein_17	DUF4113 domain-containing protein	-
E3171_RS25230	14784..15200	+	417	Protein_18	DUF1281 domain-containing protein	-
E3171_RS25235	15204..15509	+	306	WP_032163448.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	nleA/espI / stcE / exeE / exeG	1..66022	66022

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (5-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11244.27 Da
• Isoelectric Point: 10.4703

>T121176 WP_001270421.1 NZ_CP038298:c10800-10513 [Escherichia coli O157:H7]
MRYQVKFREDALKEWQKLDKTIQQQFAKKLKKCCDNPHIPSAKLRGIKDCYKIKLRASGFRLVYQVIDEQLIIAVVAVGK
RERSDVYNLASERMR

Nucleotide

• Length: 288 bp

>T121176 NZ_CP038298:c10800-10513 [Escherichia coli O157:H7]
ATGAGGTATCAGGTAAAATTCAGGGAAGATGCGCTGAAAGAGTGGCAAAAACTGGACAAGACTATTCAGCAACAGTTTGC
GAAAAAGCTAAAAAAGTGCTGTGACAATCCGCATATTCCTTCCGCAAAACTGCGCGGGATAAAGGACTGCTACAAAATAA
AATTACGTGCGTCAGGTTTTCGCCTAGTCTATCAGGTGATTGACGAACAATTAATTATCGCTGTTGTAGCTGTGGGTAAG
CGTGAGCGCAGTGACGTTTATAATCTTGCCAGTGAAAGAATGAGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9192.50 Da
• Isoelectric Point: 4.2108

>AT121176 WP_001132893.1 NZ_CP038298:c11048-10797 [Escherichia coli O157:H7]
MPNIILSDTSASVSELKKNPMATVSAGDGFPIAILNRNQPAFYCVPAELYEKMLDALDDQELVKLVAERSNQPLHDVDLD
KYL

Nucleotide

• Length: 252 bp

>AT121176 NZ_CP038298:c11048-10797 [Escherichia coli O157:H7]
ATGCCTAACATTATTCTCAGTGATACAAGCGCCAGTGTCAGCGAGCTGAAGAAAAATCCGATGGCGACAGTCAGCGCCGG
TGATGGTTTCCCGATCGCTATCCTGAACCGTAATCAGCCTGCTTTCTACTGTGTACCCGCAGAGCTGTACGAAAAGATGC
TCGATGCCCTGGACGATCAGGAGCTGGTTAAACTGGTAGCCGAACGCAGCAACCAACCGCTGCATGATGTAGACCTAGAT
AAATACCTATGA

Structures

Antitoxin

• AlphaFold DB: A0A1V0M724