Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | relBE/RHH(antitoxin) |
| Location | 74774..75333 | Replicon | plasmid pTB21-1 |
| Accession | NZ_CP038293 | ||
| Organism | Escherichia coli O157:H7 strain TB21-1 | ||
Toxin (Protein)
| Gene name | relE | Uniprot ID | O82928 |
| Locus tag | E4U65_RS27695 | Protein ID | WP_000421248.1 |
| Coordinates | 75058..75333 (+) | Length | 92 a.a. |
Antitoxin (Protein)
| Gene name | relB | Uniprot ID | C1J8K5 |
| Locus tag | E4U65_RS27690 | Protein ID | WP_001178089.1 |
| Coordinates | 74774..75058 (+) | Length | 95 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E4U65_RS27650 | 69966..70526 | + | 561 | WP_000840472.1 | fertility inhibition protein FinO | - |
| E4U65_RS27655 | 70659..70871 | + | 213 | WP_001302189.1 | ANR family transcriptional regulator | - |
| E4U65_RS27660 | 71116..71577 | + | 462 | WP_001233853.1 | thermonuclease family protein | - |
| E4U65_RS27665 | 71623..71832 | + | 210 | WP_001302200.1 | hemolysin expression modulator Hha | - |
| E4U65_RS27670 | 71870..72208 | + | 339 | WP_000766796.1 | hypothetical protein | - |
| E4U65_RS27675 | 72448..72702 | + | 255 | WP_000083831.1 | replication regulatory protein RepA | - |
| E4U65_RS27680 | 72938..73012 | + | 75 | WP_001370046.1 | RepA leader peptide Tap | - |
| E4U65_RS27685 | 73005..73862 | + | 858 | WP_000130945.1 | incFII family plasmid replication initiator RepA | - |
| E4U65_RS27690 | 74774..75058 | + | 285 | WP_001178089.1 | ribbon-helix-helix domain-containing protein | Antitoxin |
| E4U65_RS27695 | 75058..75333 | + | 276 | WP_000421248.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| E4U65_RS27700 | 75428..75634 | + | 207 | WP_001105064.1 | hypothetical protein | - |
| E4U65_RS27705 | 75734..75949 | - | 216 | Protein_82 | transposase | - |
| E4U65_RS27710 | 75993..76559 | - | 567 | Protein_83 | IS3-like element IS600 family transposase | - |
| E4U65_RS27715 | 76559..76975 | - | 417 | Protein_84 | transposase zinc-binding domain-containing protein | - |
| E4U65_RS27720 | 76975..77160 | - | 186 | WP_001302179.1 | hypothetical protein | - |
| E4U65_RS27725 | 77337..79547 | + | 2211 | WP_000592771.1 | catalase/peroxidase KatP | - |
| E4U65_RS27730 | 79591..79980 | + | 390 | WP_001172748.1 | cytochrome b562 family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | - | stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP | 1..95611 | 95611 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 92 a.a. Molecular weight: 10735.42 Da Isoelectric Point: 9.9324
>T121150 WP_000421248.1 NZ_CP038293:75058-75333 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR
Download Length: 276 bp
>T121150 NZ_CP038293:75058-75333 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG
Antitoxin
Download Length: 95 a.a. Molecular weight: 10474.89 Da Isoelectric Point: 5.6558
>AT121150 WP_001178089.1 NZ_CP038293:74774-75058 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR
Download Length: 285 bp
>AT121150 NZ_CP038293:74774-75058 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2Z6FAZ0 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A731IHA8 |