Detailed information of TA system TA121139

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 3522971..3523449
• Replicon: chromosome
• Accession: NZ_CP038292
• Organism: Escherichia coli O157:H7 strain TB21-1

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: E4U65_RS18070
• Protein ID: WP_001303876.1
• Coordinates: 3523162..3523449 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: E4U65_RS18065
• Protein ID: WP_000536233.1
• Coordinates: 3522971..3523162 (+)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U65_RS18030	3518375..3519145	-	771	WP_000450992.1	DUF1627 domain-containing protein	-
E4U65_RS18035	3519167..3519913	-	747	WP_000788751.1	ATP-binding protein	-
E4U65_RS18040	3519920..3521011	-	1092	WP_001205823.1	hypothetical protein	-
E4U65_RS18045	3521090..3521545	+	456	WP_000273724.1	hypothetical protein	-
E4U65_RS18050	3521752..3522177	-	426	WP_000693855.1	Rha family transcriptional regulator	-
E4U65_RS18055	3522161..3522433	-	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
E4U65_RS18060	3522542..3522943	+	402	WP_000986592.1	helix-turn-helix domain-containing protein	-
E4U65_RS18065	3522971..3523162	+	192	WP_000536233.1	hypothetical protein	Antitoxin
E4U65_RS18070	3523162..3523449	+	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U65_RS18075	3523727..3523882	+	156	WP_000379575.1	DUF1391 family protein	-
E4U65_RS18080	3524024..3524413	+	390	WP_000394511.1	hypothetical protein	-
E4U65_RS18085	3524600..3524785	-	186	WP_001133046.1	hypothetical protein	-
E4U65_RS18090	3525359..3525547	+	189	WP_000413705.1	cell division inhibition protein DicB	-
E4U65_RS18095	3525544..3525735	+	192	WP_001098307.1	DUF1482 family protein	-
E4U65_RS18100	3525829..3528300	+	2472	WP_000034474.1	exonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3481540..3530674	49134

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T121139 WP_001303876.1 NZ_CP038292:3523162-3523449 [Escherichia coli O157:H7]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T121139 NZ_CP038292:3523162-3523449 [Escherichia coli O157:H7]
ATGTTACCCATTTTATGGCTACCGTCTGCTCGCGATGATTTGCGCCAGATCATAACTTACATCGCCAAGGAGAACCCACC
GGCAGCACGTAGACTAAAAATACGCATTGAAACATCGGTATTACCTCTATCTGAGCATCCGTACTTATATCCACCAAGCG
AACGGGTTTCTGGATTGAGAGAGATCGTGACCCACCCTAACTACATAATCCTGTACAGAGTAGCTGCTTCAAGCATTGAG
ATTGTAAGCGTGACACATTCTCGGCGACAATTTCCCTTCTCTATCTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT121139 WP_000536233.1 NZ_CP038292:3522971-3523162 [Escherichia coli O157:H7]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT121139 NZ_CP038292:3522971-3523162 [Escherichia coli O157:H7]
ATGGGTACAGCCCTTTCTCCGATAGTTTCAGAATTCGAAACTACCGAACAAGAAAACAGTTACAACGAATGGTTGCGCAC
TAAAGTAACGTCAAGCCTTGCAGACACTCGCCCCGCAATTCCACATGACGAGGTAATGGCTGAAATGGAAAATCTTATTG
CTCAAATTGCTGTAACTAACAAGAGCGAGTAA