Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | paaR-paaA-parE/- |
| Location | 2534377..2534848 | Replicon | chromosome |
| Accession | NZ_CP038292 | ||
| Organism | Escherichia coli O157:H7 strain TB21-1 | ||
Toxin (Protein)
| Gene name | parE_1 | Uniprot ID | A0A0D7C2L1 |
| Locus tag | E4U65_RS12690 | Protein ID | WP_001303511.1 |
| Coordinates | 2534377..2534655 (-) | Length | 93 a.a. |
Antitoxin (Protein)
| Gene name | paaA | Uniprot ID | Q8XAD5 |
| Locus tag | E4U65_RS12695 | Protein ID | WP_001302048.1 |
| Coordinates | 2534657..2534848 (-) | Length | 64 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| E4U65_RS12650 | 2529383..2529567 | - | 185 | Protein_2471 | DUF3596 domain-containing protein | - |
| E4U65_RS12655 | 2529602..2529853 | - | 252 | WP_000005552.1 | excisionase family protein | - |
| E4U65_RS12660 | 2529926..2532397 | - | 2472 | WP_000048458.1 | exonuclease | - |
| E4U65_RS12665 | 2532490..2532681 | - | 192 | WP_001090200.1 | DUF1482 family protein | - |
| E4U65_RS12670 | 2532678..2532866 | - | 189 | WP_000449192.1 | cell division inhibition protein DicB | - |
| E4U65_RS12675 | 2533267..2533431 | + | 165 | WP_001331716.1 | hypothetical protein | - |
| E4U65_RS12680 | 2533435..2533653 | - | 219 | WP_001171930.1 | hypothetical protein | - |
| E4U65_RS12685 | 2533725..2534024 | - | 300 | WP_001240334.1 | hypothetical protein | - |
| E4U65_RS12690 | 2534377..2534655 | - | 279 | WP_001303511.1 | type II toxin-antitoxin system RelE/ParE family toxin | Toxin |
| E4U65_RS12695 | 2534657..2534848 | - | 192 | WP_001302048.1 | hypothetical protein | Antitoxin |
| E4U65_RS12700 | 2534869..2535240 | - | 372 | WP_001169686.1 | hypothetical protein | - |
| E4U65_RS12705 | 2535338..2535640 | + | 303 | WP_000172738.1 | transcriptional regulator | - |
| E4U65_RS12710 | 2535637..2536062 | + | 426 | WP_000693943.1 | Rha family transcriptional regulator | - |
| E4U65_RS12715 | 2536085..2537047 | + | 963 | WP_000095670.1 | helix-turn-helix domain-containing protein | - |
| E4U65_RS12720 | 2537054..2537794 | + | 741 | WP_000788936.1 | ATP-binding protein | - |
| E4U65_RS12725 | 2537820..2538589 | + | 770 | Protein_2486 | DUF1627 domain-containing protein | - |
| E4U65_RS12730 | 2538605..2539000 | + | 396 | WP_001118159.1 | DUF977 family protein | - |
| E4U65_RS12735 | 2539057..2539641 | + | 585 | WP_000206793.1 | DUF551 domain-containing protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Prophage | - | nleA/espI / espM1 / espX7/nleL / espN / espK | 2526090..2727899 | 201809 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 93 a.a. Molecular weight: 10661.25 Da Isoelectric Point: 5.5647
>T121128 WP_001303511.1 NZ_CP038292:c2534655-2534377 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP
Download Length: 279 bp
>T121128 NZ_CP038292:c2534655-2534377 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA
Antitoxin
Download Length: 64 a.a. Molecular weight: 7463.47 Da Isoelectric Point: 8.6828
>AT121128 WP_001302048.1 NZ_CP038292:c2534848-2534657 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ
Download Length: 192 bp
>AT121128 NZ_CP038292:c2534848-2534657 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|