Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 359371..360002 Replicon chromosome
Accession NZ_CP038292
Organism Escherichia coli O157:H7 strain TB21-1

Toxin (Protein)


Gene name hicA Uniprot ID U9Y2A7
Locus tag E4U65_RS01645 Protein ID WP_001259385.1
Coordinates 359371..359646 (+) Length 92 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID U9Y7E1
Locus tag E4U65_RS01650 Protein ID WP_000593555.1
Coordinates 359643..360002 (+) Length 120 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4U65_RS01630 354375..355442 + 1068 WP_000361458.1 HlyD family secretion protein -
E4U65_RS01635 355439..358174 + 2736 WP_000149107.1 ribosome-associated ATPase/putative transporter RbbA -
E4U65_RS01640 358174..359298 + 1125 WP_001301659.1 ABC transporter permease -
E4U65_RS01645 359371..359646 + 276 WP_001259385.1 type II toxin-antitoxin system HicA family toxin Toxin
E4U65_RS01650 359643..360002 + 360 WP_000593555.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E4U65_RS01655 360052..360912 - 861 WP_001131788.1 class II aldolase -
E4U65_RS01660 360944..361213 - 270 WP_000933060.1 HPr family phosphocarrier protein -
E4U65_RS01665 361203..362711 - 1509 WP_001301773.1 carbohydrate kinase -
E4U65_RS01670 362704..364062 - 1359 WP_001302220.1 PTS galactitol transporter subunit IIC -
E4U65_RS01675 364139..364420 - 282 WP_000084025.1 PTS sugar transporter subunit IIB -
E4U65_RS01680 364417..364890 - 474 WP_001161553.1 PTS sugar transporter subunit IIA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - - 350515..360002 9487


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(30-80)

Antitoxin

Uncharacterised protein family HicB

(60-112)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10199.94 Da        Isoelectric Point: 10.7076

>T121112 WP_001259385.1 NZ_CP038292:359371-359646 [Escherichia coli O157:H7]
MRTKVQALRKKQKNTLDQIFKTPVPQGIKWSDIESLVKALGGEIKEGRGSRCKFILNMSVACFHRPHPSPDTDKGAVESV
RDWLLSIGVKP

Download         Length: 276 bp

>T121112 NZ_CP038292:359371-359646 [Escherichia coli O157:H7]
ATGAGAACCAAGGTACAGGCTTTACGGAAGAAACAAAAAAATACATTGGATCAAATATTTAAAACTCCTGTTCCTCAAGG
GATCAAGTGGTCTGATATAGAGTCACTGGTTAAAGCATTAGGCGGAGAAATTAAGGAAGGAAGAGGTTCGCGTTGTAAGT
TCATACTAAATATGAGCGTTGCGTGTTTCCATCGGCCTCATCCGTCGCCAGATACCGATAAAGGCGCTGTAGAAAGCGTG
CGTGACTGGTTACTAAGTATAGGGGTAAAACCATGA

Antitoxin


Download         Length: 120 a.a.        Molecular weight: 13402.27 Da        Isoelectric Point: 5.1987

>AT121112 WP_000593555.1 NZ_CP038292:359643-360002 [Escherichia coli O157:H7]
MIKLKTPNSMEIAGQPAVITYVPELNAFRGKFLGLSGYCDFVSDSIQGLQKEGELSLREYLEDCKAAGIEPYARTEKIKT
FTLRYPESLSERLNNAAAQQQVSVNTYIIETLNERLNHL

Download         Length: 360 bp

>AT121112 NZ_CP038292:359643-360002 [Escherichia coli O157:H7]
ATGATCAAACTGAAGACGCCAAACTCTATGGAGATTGCCGGGCAACCTGCTGTTATCACTTACGTGCCAGAACTGAATGC
ATTTCGTGGCAAATTCCTGGGCTTGTCAGGTTATTGTGATTTTGTCTCTGACAGCATTCAGGGGCTGCAAAAAGAAGGGG
AACTTTCCCTGCGAGAGTATCTTGAGGATTGTAAAGCTGCGGGTATTGAGCCGTATGCGCGTACAGAAAAGATTAAGACC
TTTACGTTACGCTATCCAGAATCGCTGAGTGAACGCCTTAATAATGCGGCTGCGCAGCAACAAGTCTCTGTTAATACCTA
TATTATTGAAACACTGAATGAGCGTTTGAATCATTTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TTW8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9LKZ6

References