Detailed information of TA system TA121064

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RHH(antitoxin)
• Location: 74768..75327
• Replicon: plasmid pTX376-1
• Accession: NZ_CP038289
• Organism: Escherichia coli O157:H7 strain TX 376-2

Toxin (Protein)

• Gene name: relE
• Uniprot ID: O82928
• Locus tag: E4U64_RS27965
• Protein ID: WP_000421248.1
• Coordinates: 75052..75327 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: C1J8K5
• Locus tag: E4U64_RS27960
• Protein ID: WP_001178089.1
• Coordinates: 74768..75052 (+)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U64_RS27920	69960..70520	+	561	WP_000840472.1	fertility inhibition protein FinO	-
E4U64_RS27925	70653..70865	+	213	WP_001302189.1	ANR family transcriptional regulator	-
E4U64_RS27930	71110..71571	+	462	WP_001233853.1	thermonuclease family protein	-
E4U64_RS27935	71617..71826	+	210	WP_001302200.1	hemolysin expression modulator Hha	-
E4U64_RS27940	71864..72202	+	339	WP_000766796.1	hypothetical protein	-
E4U64_RS27945	72442..72696	+	255	WP_000083831.1	replication regulatory protein RepA	-
E4U64_RS27950	72932..73006	+	75	WP_001370046.1	RepA leader peptide Tap	-
E4U64_RS27955	72999..73856	+	858	WP_000130945.1	incFII family plasmid replication initiator RepA	-
E4U64_RS27960	74768..75052	+	285	WP_001178089.1	ribbon-helix-helix domain-containing protein	Antitoxin
E4U64_RS27965	75052..75327	+	276	WP_000421248.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U64_RS27970	75422..75628	+	207	WP_001105064.1	hypothetical protein	-
E4U64_RS27975	75728..75943	-	216	Protein_82	transposase	-
E4U64_RS27980	75987..76553	-	567	Protein_83	IS3-like element IS600 family transposase	-
E4U64_RS27985	76553..76969	-	417	Protein_84	transposase zinc-binding domain-containing protein	-
E4U64_RS27990	77128..78341	+	1214	WP_162829202.1	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	stcE / exeE / exeG / hlyC / hlyA / hlyB / hlyD / toxB / espP	1..96920	96920

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (3-90)

Antitoxin

• Ribbon-helix-helix protein, CopG (14-51)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10735.42 Da
• Isoelectric Point: 9.9324

>T121064 WP_000421248.1 NZ_CP038289:75052-75327 [Escherichia coli O157:H7]
MELKWSSKALSDLARLYDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYELNGQTIY
VLRLWHTRENR

Nucleotide

• Length: 276 bp

>T121064 NZ_CP038289:75052-75327 [Escherichia coli O157:H7]
ATGGAACTGAAGTGGAGCAGTAAGGCGCTTTCTGATTTGGCGCGGTTATATGATTTTCTGGTGCTAGCCAGTAAACCTGC
TGCCGCCAGAACGGTACAGTCCCTGACACAGGCACCGGTCATTCTGTTAACTCATCCACGTATGGGGGAACAGTTATTTC
AGTTTGAACCCAGGGAGGTCAGACGGATTTTTGCTGGCGAGTACGAAATCCGTTACGAACTTAATGGCCAGACTATTTAT
GTATTGCGCCTGTGGCACACACGAGAAAACAGGTAG

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10474.89 Da
• Isoelectric Point: 5.6558

>AT121064 WP_001178089.1 NZ_CP038289:74768-75052 [Escherichia coli O157:H7]
MQMKNNTAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
ADSLSTDNPLPVPR

Nucleotide

• Length: 285 bp

>AT121064 NZ_CP038289:74768-75052 [Escherichia coli O157:H7]
ATGCAAATGAAAAACAATACCGCACAAGCAACAAAAGTCATTACCGCGCATGTGCCATTACCTATGGCTGATAAAGTCGA
CCAGATGGCCGCCAGACTGGAACGCTCCCGGGGTTGGGTTATCAAACAGGCGCTTTCTGCATGGCTTGCCCAGGAGGAGG
AGCGTAATCGCCTGACGCTGGAAGCCCTGGACGATGTGACATCCGGACAGGTTATTGACCATCAGGCTGTACAGGCCTGG
GCGGACAGCCTCAGTACTGACAATCCGTTACCGGTGCCACGCTGA

Structures

Toxin

• AlphaFold DB: A0A2Z6FAZ0

Antitoxin

• AlphaFold DB: A0A731IHA8