Detailed information of TA system TA121062

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-Phd
• Location: 64214..64815
• Replicon: plasmid pTX376-2
• Accession: NZ_CP038288
• Organism: Escherichia coli O157:H7 strain TX 376-2

Toxin (Protein)

• Gene name: doc
• Uniprot ID: F0JYA5
• Locus tag: E4U64_RS27305
• Protein ID: WP_001216044.1
• Coordinates: 64214..64594 (-)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: U9YQH9
• Locus tag: E4U64_RS27310
• Protein ID: WP_001190712.1
• Coordinates: 64594..64815 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U64_RS27280	59655..61139	-	1485	WP_000124159.1	hypothetical protein	-
E4U64_RS27285	61139..62332	-	1194	WP_023442315.1	hypothetical protein	-
E4U64_RS27290	62418..62870	-	453	WP_001326849.1	late promoter-activating protein	-
E4U64_RS27295	62959..64002	-	1044	WP_023442316.1	DUF968 domain-containing protein	-
E4U64_RS27300	64030..64209	-	180	WP_024224144.1	hypothetical protein	-
E4U64_RS27305	64214..64594	-	381	WP_001216044.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
E4U64_RS27310	64594..64815	-	222	WP_001190712.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E4U64_RS27315	64888..65277	-	390	WP_024224146.1	DNA repair protein	-
E4U64_RS27320	65452..66024	+	573	WP_032353386.1	hypothetical protein	-
E4U64_RS27325	66031..66282	-	252	WP_024224114.1	DNA polymerase III subunit theta	-
E4U64_RS27330	66597..66857	-	261	WP_001408981.1	hypothetical protein	-
E4U64_RS27335	67130..67780	-	651	WP_000057453.1	hypothetical protein	-
E4U64_RS27340	67762..68136	-	375	WP_024224113.1	hypothetical protein	-
E4U64_RS27345	68143..68436	-	294	WP_000269004.1	hypothetical protein	-
E4U64_RS27350	68615..68848	-	234	WP_000516537.1	hypothetical protein	-
E4U64_RS27355	68934..69194	-	261	WP_024224112.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..99810	99810

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (2-123)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-51)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 13544.28 Da
• Isoelectric Point: 5.6343

>T121062 WP_001216044.1 NZ_CP038288:c64594-64214 [Escherichia coli O157:H7]
MRHISPEELIALHDANISRYGGLPGMSDPGRAEAIIGRVQARVAYEEITDLFEVSATYLVATARGHIFNDANKRTALNSA
LLFLRRNGVQVFDSPELADLTVGAATGEISVSSVAATLRRLYGSAE

Nucleotide

• Length: 381 bp

>T121062 NZ_CP038288:c64594-64214 [Escherichia coli O157:H7]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAGCCGCTACGGCGGCCTGCCGGGAATGTC
AGATCCGGGTAGGGCAGAGGCCATTATCGGGAGAGTTCAGGCCAGAGTTGCCTACGAAGAGATCACCGACCTTTTCGAAG
TCTCCGCCACCTACCTGGTGGCTACGGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACTGG
CGAGATATCTGTATCTTCTGTCGCCGCCACGTTACGTAGATTGTATGGTTCTGCGGAGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8133.10 Da
• Isoelectric Point: 4.7708

>AT121062 WP_001190712.1 NZ_CP038288:c64815-64594 [Escherichia coli O157:H7]
MQSINFRTARGNLSEVLNNVEAGEEVEITRRGREPAVIVSKATFEAYKKAALDAEFASLFDTLDSTNKELVNR

Nucleotide

• Length: 222 bp

>AT121062 NZ_CP038288:c64815-64594 [Escherichia coli O157:H7]
ATGCAATCCATTAACTTCCGTACCGCGCGTGGCAACCTTTCTGAAGTGCTTAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A6Y6HMN5

Antitoxin

• AlphaFold DB: A0A829CJB6