Detailed information of TA system TA121043

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2523291..2523762
• Replicon: chromosome
• Accession: NZ_CP038287
• Organism: Escherichia coli O157:H7 strain TX 376-2

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E4U64_RS12580
• Protein ID: WP_001303511.1
• Coordinates: 2523291..2523569 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E4U64_RS12585
• Protein ID: WP_001302048.1
• Coordinates: 2523571..2523762 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4U64_RS12540	2518297..2518481	-	185	Protein_2449	DUF3596 domain-containing protein	-
E4U64_RS12545	2518516..2518767	-	252	WP_000005552.1	excisionase family protein	-
E4U64_RS12550	2518840..2521311	-	2472	WP_000048458.1	exonuclease	-
E4U64_RS12555	2521404..2521595	-	192	WP_001090200.1	DUF1482 family protein	-
E4U64_RS12560	2521592..2521780	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E4U64_RS12565	2522181..2522345	+	165	WP_001331716.1	hypothetical protein	-
E4U64_RS12570	2522349..2522567	-	219	WP_001171930.1	hypothetical protein	-
E4U64_RS12575	2522639..2522938	-	300	WP_001240334.1	hypothetical protein	-
E4U64_RS12580	2523291..2523569	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4U64_RS12585	2523571..2523762	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E4U64_RS12590	2523783..2524154	-	372	WP_001169686.1	hypothetical protein	-
E4U64_RS12595	2524252..2524554	+	303	WP_000172738.1	transcriptional regulator	-
E4U64_RS12600	2524551..2524976	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E4U64_RS12605	2524999..2525961	+	963	WP_000095670.1	helix-turn-helix domain-containing protein	-
E4U64_RS12610	2525968..2526708	+	741	WP_000788936.1	ATP-binding protein	-
E4U64_RS12615	2526734..2527503	+	770	Protein_2464	DUF1627 domain-containing protein	-
E4U64_RS12620	2527519..2527914	+	396	WP_001118159.1	DUF977 family protein	-
E4U64_RS12625	2527971..2528555	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI / espM1 / nleG7'	2515004..2679267	164263

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T121043 WP_001303511.1 NZ_CP038287:c2523569-2523291 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T121043 NZ_CP038287:c2523569-2523291 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT121043 WP_001302048.1 NZ_CP038287:c2523762-2523571 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT121043 NZ_CP038287:c2523762-2523571 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0