Detailed information of TA system    

experimentalExperimentally validated    

Overview


TA module


Type II Classification (family/domain) vapBC/RHH-PIN
Location 3070170..3070849 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name vapC Uniprot ID P9WF91
Locus tag Rv2757c Protein ID NP_217273.1
Coordinates 3070170..3070586 (-) Length 139 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ43
Locus tag Rv2758c Protein ID NP_217274.1
Coordinates 3070583..3070849 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv2753c 3066222..3067124 - 903 NP_217269.1 4-hydroxy-tetrahydrodipicolinate synthase -
Rv2754c 3067193..3067945 - 753 NP_217270.1 thymidylate synthase ThyX -
Rv2756c 3068461..3070083 - 1623 NP_217272.1 type I restriction/modification system DNA methylase HsdM -
Rv2757c 3070170..3070586 - 417 NP_217273.1 ribonuclease VapC21 Toxin
Rv2758c 3070583..3070849 - 267 NP_217274.1 antitoxin VapB21 Antitoxin
Rv2759c 3070875..3071270 - 396 NP_217275.1 ribonuclease VapC42 -
Rv2760c 3071267..3071536 - 270 NP_217276.1 antitoxin VapB42 -
Rv2761c 3071546..3072640 - 1095 NP_217277.1 type I restriction/modification system specificity determinant HsdS -
Rv2762c 3072637..3073056 - 420 NP_217278.1 hypothetical protein -
Rv2763c 3073130..3073609 - 480 NP_217279.1 dihydrofolate reductase -
Rv2764c 3073680..3074471 - 792 NP_217280.1 thymidylate synthase ThyA -
Rv2765 3074636..3075373 + 738 NP_217281.1 hydrolase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(5-123)

Antitoxin

(16-56)


Sequences


Toxin        


Download         Length: 139 a.a.        Molecular weight: 15773.00 Da        Isoelectric Point: 7.1294

>T121 NP_217273.1 NC_000962:c3070586-3070170 [Mycobacterium tuberculosis H37Rv]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Download         Length: 417 bp

>T121 NC_000962:c3070586-3070170 [Mycobacterium tuberculosis H37Rv]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9493.77 Da        Isoelectric Point: 7.2783

>AT121 NP_217274.1 NC_000962:c3070849-3070583 [Mycobacterium tuberculosis H37Rv]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Download         Length: 267 bp

>AT121 NC_000962:c3070849-3070583 [Mycobacterium tuberculosis H37Rv]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
T74 Mycobacterium tuberculosis H37Rv

42.424

95.652

0.406


Multiple sequence alignment    


Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
PDB 5SV2


Antitoxin

Source ID Structure
AlphaFold DB P9WJ43

References


(1) Uddipan Das et al. (2014) Crystal structure of the VapBC-15 complex from Mycobacterium tuberculosis reveals a two-metal ion dependent PIN-domain ribonuclease and a variable mode of toxin-antitoxin assembly. Journal of Structural Biology 188(3):249-58. [PubMed:25450593] experimental

(2) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113] experimental

(3) Arun Sharma et al. (2020) VapC21 Toxin Contributes to Drug-Tolerance and Interacts With Non-cognate VapB32 Antitoxin in Mycobacterium tuberculosis. Frontiers in Microbiology 11:2037. [PubMed:33042034] experimental


experimental experimental literature