Detailed information of TA system TA120969

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 2602797..2603268
• Replicon: chromosome
• Accession: NZ_CP038282
• Organism: Escherichia coli O157:H7 strain F8492

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A0D7C2L1
• Locus tag: E4T53_RS13195
• Protein ID: WP_001303511.1
• Coordinates: 2602797..2603075 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XAD5
• Locus tag: E4T53_RS13200
• Protein ID: WP_001302048.1
• Coordinates: 2603077..2603268 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4T53_RS13155	2597803..2597987	-	185	Protein_2575	DUF3596 domain-containing protein	-
E4T53_RS13160	2598022..2598273	-	252	WP_000005552.1	excisionase family protein	-
E4T53_RS13165	2598346..2600817	-	2472	WP_000048458.1	exonuclease	-
E4T53_RS13170	2600910..2601101	-	192	WP_001090200.1	DUF1482 family protein	-
E4T53_RS13175	2601098..2601286	-	189	WP_000449192.1	cell division inhibition protein DicB	-
E4T53_RS13180	2601687..2601851	+	165	WP_001331716.1	hypothetical protein	-
E4T53_RS13185	2601855..2602073	-	219	WP_171878004.1	hypothetical protein	-
E4T53_RS13190	2602145..2602444	-	300	WP_001240334.1	hypothetical protein	-
E4T53_RS13195	2602797..2603075	-	279	WP_001303511.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4T53_RS13200	2603077..2603268	-	192	WP_001302048.1	hypothetical protein	Antitoxin
E4T53_RS13205	2603289..2603660	-	372	WP_001169686.1	hypothetical protein	-
E4T53_RS13210	2603758..2604060	+	303	WP_000172738.1	transcriptional regulator	-
E4T53_RS13215	2604057..2604482	+	426	WP_000693943.1	Rha family transcriptional regulator	-
E4T53_RS13220	2604505..2605467	+	963	WP_000095669.1	helix-turn-helix domain-containing protein	-
E4T53_RS13225	2605474..2606214	+	741	WP_000788938.1	ATP-binding protein	-
E4T53_RS13230	2606240..2607009	+	770	Protein_2590	DUF1627 domain-containing protein	-
E4T53_RS13235	2607025..2607420	+	396	WP_001118159.1	DUF977 family protein	-
E4T53_RS13240	2607477..2608061	+	585	WP_000206793.1	DUF551 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleA/espI	2587917..2643924	56007

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10661.25 Da
• Isoelectric Point: 5.5647

>T120969 WP_001303511.1 NZ_CP038282:c2603075-2602797 [Escherichia coli O157:H7]
MLPVLWLESADTDLDDITSYIARFDIDAAERLWQRLRGCVLPLSEHPYLYPPSDRVPGLREIVAHPNYIILYRVTTSSVE
VVNVIHARRQFP

Nucleotide

• Length: 279 bp

>T120969 NZ_CP038282:c2603075-2602797 [Escherichia coli O157:H7]
ATGTTACCCGTGTTATGGCTTGAAAGCGCAGATACCGACCTAGATGATATAACTAGTTATATTGCTCGTTTCGACATAGA
TGCGGCTGAACGCTTATGGCAGCGATTAAGGGGTTGTGTGCTGCCGTTATCCGAACATCCGTATTTATACCCACCAAGCG
ACAGAGTACCTGGCTTGCGTGAGATTGTAGCCCACCCTAACTATATAATTCTATACCGCGTAACAACATCAAGCGTTGAA
GTAGTAAACGTGATCCACGCAAGACGCCAGTTTCCCTAA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7463.47 Da
• Isoelectric Point: 8.6828

>AT120969 WP_001302048.1 NZ_CP038282:c2603268-2603077 [Escherichia coli O157:H7]
MNRALSPMVSEFETIEQENSYNEWLRAKVATSLADPRPAIPHDEVERRMAERFAKMRKERSKQ

Nucleotide

• Length: 192 bp

>AT120969 NZ_CP038282:c2603268-2603077 [Escherichia coli O157:H7]
ATGAATAGAGCCCTTTCACCAATGGTTTCTGAATTTGAAACCATTGAACAAGAAAACAGTTACAACGAATGGCTGCGTGC
GAAAGTAGCAACGAGCCTTGCAGATCCGCGCCCAGCAATTCCCCATGACGAAGTTGAGCGCAGAATGGCAGAACGCTTTG
CTAAGATGCGCAAGGAACGGAGCAAGCAGTAA

Structures

Toxin

• PDB: 5CZF
• PDB: 5CW7
• PDB: 5CZE

Antitoxin

• PDB: 5CZF
• PDB: 3ZBE
• PDB: 5CW7
• AlphaFold DB: A0A829CTP0