Detailed information of TA system TA120950

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 5307382..5308133
• Replicon: chromosome
• Accession: NZ_CP038281
• Organism: Raoultella ornithinolytica strain WLK218

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: E4K08_RS28930
• Protein ID: WP_044351088.1
• Coordinates: 5307382..5307864 (-)
• Length: 161 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A808Y4Y8
• Locus tag: E4K08_RS28935
• Protein ID: WP_015584326.1
• Coordinates: 5307855..5308133 (-)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K08_RS28905	5302662..5304065	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
E4K08_RS28910	5304094..5304726	-	633	WP_001567369.1	hypothetical protein	-
E4K08_RS28915	5304952..5306298	+	1347	WP_137056603.1	ISNCY family transposase	-
E4K08_RS28920	5306347..5306751	+	405	WP_137056604.1	helix-turn-helix domain-containing protein	-
E4K08_RS30775	5306847..5307197	-	351	WP_044351090.1	hypothetical protein	-
E4K08_RS28930	5307382..5307864	-	483	WP_044351088.1	GNAT family N-acetyltransferase	Toxin
E4K08_RS28935	5307855..5308133	-	279	WP_015584326.1	DUF1778 domain-containing protein	Antitoxin
E4K08_RS28940	5308614..5309111	-	498	WP_137056693.1	hypothetical protein	-
E4K08_RS28945	5309302..5309999	+	698	Protein_4884	IS1 family transposase	-
E4K08_RS28950	5310104..5311978	-	1875	WP_071359406.1	KUP/HAK/KT family potassium transporter	-
E4K08_RS28955	5312002..5312547	-	546	WP_044351084.1	DUF1003 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	5145963..5336279	190316
	-	inside	IScluster/Tn	-	-	5299043..5313744	14701

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-144)

Antitoxin

• Vibrio phage ICP1, Orf50 (3-92)

Sequences

Toxin

Protein

• Length: 161 a.a.
• Molecular weight: 17612.48 Da
• Isoelectric Point: 9.7381

>T120950 WP_044351088.1 NZ_CP038281:c5307864-5307382 [Raoultella ornithinolytica]
MGMRPPEPLSAGHNIAEFCCQDQVLNEWLKKKALKNHSTGISRVFVVCAENTKRVIAYYSLSSGSVHRNTVPGAYRRNAP
EAVPVIVLGRLAVDAAWAGKGLGAALLKDAIYRTEHIAVQVGVRALLVHALNDEVREFYTRFGFEPSIINTLTLLFPIKV

Nucleotide

• Length: 483 bp

>T120950 NZ_CP038281:c5307864-5307382 [Raoultella ornithinolytica]
ATGGGAATGAGACCACCGGAACCACTCAGCGCTGGACACAACATAGCAGAATTTTGTTGTCAGGATCAGGTTCTTAACGA
GTGGTTGAAGAAGAAGGCGCTGAAGAACCACAGTACAGGCATTTCAAGGGTCTTTGTGGTCTGTGCCGAAAACACAAAAA
GGGTCATCGCGTATTACAGCTTGTCATCCGGGAGCGTGCACAGAAACACAGTACCGGGGGCATACCGTCGTAATGCACCT
GAAGCTGTACCGGTCATTGTTCTGGGAAGGCTTGCGGTCGATGCAGCCTGGGCAGGGAAGGGACTCGGAGCGGCACTGCT
TAAGGATGCAATATACCGTACAGAACATATCGCAGTTCAGGTCGGTGTAAGGGCGCTTCTCGTCCATGCCTTGAATGATG
AAGTCAGGGAGTTTTACACCAGGTTTGGATTCGAGCCCTCGATTATCAATACACTGACATTATTGTTCCCGATAAAAGTA
TGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10714.30 Da
• Isoelectric Point: 4.6722

>AT120950 WP_015584326.1 NZ_CP038281:c5308133-5307855 [Raoultella ornithinolytica]
MKPDVREAPINIRAKTSQRDLIDMAANLVSKSRTDFMLEVACREAQDILLDQRLFLLDDKQFASFMEELDAPMTAERQAR
IDNLMNRKSPWE

Nucleotide

• Length: 279 bp

>AT120950 NZ_CP038281:c5308133-5307855 [Raoultella ornithinolytica]
ATGAAACCAGACGTTCGTGAAGCTCCGATAAACATTCGGGCGAAAACATCTCAGCGCGACCTTATCGACATGGCCGCAAA
TCTTGTATCTAAATCACGTACTGATTTTATGCTTGAAGTGGCCTGTCGCGAAGCTCAGGACATTCTGTTGGATCAGCGCT
TGTTCCTGCTGGACGATAAGCAGTTTGCTAGTTTCATGGAAGAGCTTGACGCACCTATGACAGCGGAGCGTCAGGCACGT
ATTGATAACCTGATGAACCGCAAATCGCCATGGGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A808Y4Y8