Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3813194..3813813 Replicon chromosome
Accession NZ_CP038281
Organism Raoultella ornithinolytica strain WLK218

Toxin (Protein)


Gene name Hha Uniprot ID A0A514EV16
Locus tag E4K08_RS21790 Protein ID WP_004858783.1
Coordinates 3813194..3813412 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A514EV88
Locus tag E4K08_RS21795 Protein ID WP_004858785.1
Coordinates 3813439..3813813 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4K08_RS21760 3809266..3809526 + 261 WP_004858777.1 type B 50S ribosomal protein L31 -
E4K08_RS21765 3809529..3809669 + 141 WP_003859006.1 type B 50S ribosomal protein L36 -
E4K08_RS21770 3809666..3810376 - 711 WP_015585015.1 GNAT family N-acetyltransferase -
E4K08_RS21775 3810480..3811931 + 1452 WP_069476198.1 PLP-dependent aminotransferase family protein -
E4K08_RS21780 3811906..3812376 - 471 WP_128318549.1 YlaC family protein -
E4K08_RS21785 3812476..3813042 - 567 WP_004858781.1 maltose O-acetyltransferase -
E4K08_RS21790 3813194..3813412 - 219 WP_004858783.1 hemolysin expression modulator Hha Toxin
E4K08_RS21795 3813439..3813813 - 375 WP_004858785.1 Hha toxicity modulator TomB Antitoxin
E4K08_RS21800 3814341..3817487 - 3147 WP_137056373.1 multidrug efflux RND transporter permease subunit AcrB -
E4K08_RS21805 3817510..3818703 - 1194 WP_004858789.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-116)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8570.90 Da        Isoelectric Point: 7.9907

>T120948 WP_004858783.1 NZ_CP038281:c3813412-3813194 [Raoultella ornithinolytica]
MSGEPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPTSVWKFIR

Download         Length: 219 bp

>T120948 NZ_CP038281:c3813412-3813194 [Raoultella ornithinolytica]
ATGTCTGGTGAACCATTAACCAAAACAGACTATTTGATGCGCTTACGTCGCTGTCAGACAATTGATACGCTGGAACGCGT
AATTGAAAAAAACAAATATGAACTTTCAGATAACGAACTGGCTGTATTTTACTCAGCGGCCGACCACCGTCTTGCTGAAT
TGACCATGAATAAGCTCTACGACAAAATCCCGACATCGGTGTGGAAGTTCATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14411.19 Da        Isoelectric Point: 4.8989

>AT120948 WP_004858785.1 NZ_CP038281:c3813813-3813439 [Raoultella ornithinolytica]
MDEYSPKRHDIAQLRFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYAEDNKLIAQLDEYL
DDTFMLFSSYGINTADLQKWRKSGNRLFRCFVNASRENPASLSC

Download         Length: 375 bp

>AT120948 NZ_CP038281:c3813813-3813439 [Raoultella ornithinolytica]
ATGGACGAATACTCGCCAAAAAGACATGATATCGCGCAGCTTCGATTTCTTTGCGAAACCCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGTAACCACGGGTGGGTGAATGATCCAACGTCGGCTATCAATCTTCAACTTAATGAATTGATCGAAC
ACATAGCCACGTTCGCGCTTAATTATAAAATTAAGTATGCTGAGGATAATAAACTGATCGCTCAGCTGGACGAATACCTG
GATGACACTTTTATGTTGTTCAGCAGTTATGGCATTAACACGGCCGATCTACAGAAATGGCGCAAATCGGGAAATCGGTT
ATTCCGATGTTTTGTCAACGCCAGTCGGGAAAATCCGGCAAGTCTTTCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A514EV16

Antitoxin

Source ID Structure
AlphaFold DB A0A514EV88

References