Detailed information of TA system TA120947

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): shpAB/BrnT-BrnA
• Location: 3232343..3232910
• Replicon: chromosome
• Accession: NZ_CP038281
• Organism: Raoultella ornithinolytica strain WLK218

Toxin (Protein)

• Gene name: shpA
• Uniprot ID: -
• Locus tag: E4K08_RS18990
• Protein ID: WP_075808908.1
• Coordinates: 3232343..3232618 (+)
• Length: 92 a.a.

Antitoxin (Protein)

• Gene name: shpB
• Uniprot ID: -
• Locus tag: E4K08_RS18995
• Protein ID: WP_004857518.1
• Coordinates: 3232605..3232910 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K08_RS18960	3227838..3228356	-	519	WP_004857491.1	hypothetical protein	-
E4K08_RS18965	3228353..3229165	-	813	WP_004857492.1	winged helix-turn-helix domain-containing protein	-
E4K08_RS18970	3229309..3229887	-	579	WP_004857495.1	TetR/AcrR family transcriptional regulator	-
E4K08_RS18975	3230035..3230817	-	783	WP_137056308.1	NAD(P)H-dependent oxidoreductase	-
E4K08_RS18980	3230905..3231813	+	909	WP_004857513.1	LysR family transcriptional regulator	-
E4K08_RS18985	3231807..3232226	+	420	WP_015585224.1	FosA family fosfomycin resistance glutathione transferase	-
E4K08_RS18990	3232343..3232618	+	276	WP_075808908.1	BrnT family toxin	Toxin
E4K08_RS18995	3232605..3232910	+	306	WP_004857518.1	BrnA antitoxin family protein	Antitoxin
E4K08_RS19000	3233064..3234476	-	1413	WP_015585221.1	PLP-dependent aminotransferase family protein	-
E4K08_RS19005	3234654..3234818	+	165	WP_004857521.1	DUF1127 domain-containing protein	-
E4K08_RS19010	3235109..3235573	+	465	WP_004857523.1	MarR family transcriptional regulator	-
E4K08_RS19015	3235580..3236647	+	1068	WP_004857525.1	HlyD family secretion protein	-
E4K08_RS19020	3236637..3237701	+	1065	WP_004857527.1	DUF2955 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-87)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (35-93)

Sequences

Toxin

Protein

• Length: 92 a.a.
• Molecular weight: 10588.93 Da
• Isoelectric Point: 6.7598

>T120947 WP_075808908.1 NZ_CP038281:3232343-3232618 [Raoultella ornithinolytica]
MPMEFEWDANKAASNLRKHGIRFEEAVLVFDDPQHLSRHDRYENGEYRWQTIGLVHGVIVVLVAHSIRFESGTEVIRIIS
ARKADKNYEHG

Nucleotide

• Length: 276 bp

>T120947 NZ_CP038281:3232343-3232618 [Raoultella ornithinolytica]
ATGCCAATGGAGTTTGAATGGGATGCAAACAAGGCAGCAAGCAATCTAAGAAAACATGGGATTCGCTTCGAGGAGGCGGT
ACTGGTTTTTGACGATCCGCAGCATCTTTCCCGACATGATCGCTATGAAAATGGTGAGTATCGATGGCAAACCATCGGGC
TTGTTCATGGCGTGATTGTTGTTCTTGTGGCGCATTCGATCCGATTTGAAAGTGGAACGGAGGTCATTCGTATTATCAGC
GCCCGGAAGGCGGACAAAAATTATGAGCATGGTTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11681.22 Da
• Isoelectric Point: 9.4429

>AT120947 WP_004857518.1 NZ_CP038281:3232605-3232910 [Raoultella ornithinolytica]
MSMVKHKRDNPTALTAQREDELKALAKKSDDDIDYSDIPSSDDEPWSEAVRGKFYRPLKTQASVRIDADVMEWLKRPGKG
YQTRLNAILREAMIREQNKKS

Nucleotide

• Length: 306 bp

>AT120947 NZ_CP038281:3232605-3232910 [Raoultella ornithinolytica]
ATGAGCATGGTTAAACATAAACGCGATAACCCGACAGCGCTTACCGCTCAGCGTGAAGACGAACTCAAAGCGCTGGCGAA
AAAATCAGATGATGACATTGATTACAGCGATATCCCCTCTTCGGACGATGAGCCGTGGTCAGAGGCGGTTCGTGGTAAAT
TTTATCGGCCATTAAAAACGCAGGCCTCTGTGCGCATCGATGCAGATGTGATGGAGTGGCTTAAACGCCCGGGAAAAGGC
TATCAAACGCGGCTGAATGCGATTTTAAGAGAAGCGATGATTCGCGAGCAGAATAAGAAATCGTAG