Detailed information of TA system TA120946

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 2316282..2316829
• Replicon: chromosome
• Accession: NZ_CP038281
• Organism: Raoultella ornithinolytica strain WLK218

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: E4K08_RS14595
• Protein ID: WP_088883551.1
• Coordinates: 2316282..2316590 (-)
• Length: 103 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A808XYT1
• Locus tag: E4K08_RS14600
• Protein ID: WP_004869059.1
• Coordinates: 2316593..2316829 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K08_RS14565	2311461..2311763	-	303	WP_004869065.1	PTS lactose/cellobiose transporter subunit IIA	-
E4K08_RS14570	2311898..2313223	-	1326	WP_004869064.1	PTS cellobiose transporter subunit IIC	-
E4K08_RS14575	2313235..2313549	-	315	WP_004869063.1	PTS sugar transporter subunit IIB	-
E4K08_RS14580	2313844..2314785	+	942	WP_015585487.1	LacI family transcriptional regulator	-
E4K08_RS14585	2314880..2315155	-	276	WP_160873538.1	hypothetical protein	-
E4K08_RS14590	2315264..2316166	-	903	WP_015585496.1	EamA family transporter	-
E4K08_RS14595	2316282..2316590	-	309	WP_088883551.1	CcdB family protein	Toxin
E4K08_RS14600	2316593..2316829	-	237	WP_004869059.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
E4K08_RS14605	2316935..2318368	-	1434	WP_004869058.1	PTS N-acetylmuramic acid transporter subunit IIBC	-
E4K08_RS14610	2318393..2319295	-	903	WP_044350979.1	N-acetylmuramic acid 6-phosphate etherase	-
E4K08_RS14615	2319460..2320623	-	1164	WP_004869055.1	multidrug effflux MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-101)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 103 a.a.
• Molecular weight: 11794.61 Da
• Isoelectric Point: 6.7154

>T120946 WP_088883551.1 NZ_CP038281:c2316590-2316282 [Raoultella ornithinolytica]
IQYYVYKNTGRITVYPYLLDVQSDIIGMRNTRVVIPLFPLRNYKGPRADRLTPVVTVEGEEYVVMTHELASIPQRVLGEE
VCHLNHQREVVKASIDFLYDGI

Nucleotide

• Length: 309 bp

>T120946 NZ_CP038281:c2316590-2316282 [Raoultella ornithinolytica]
ATACAGTATTACGTTTATAAAAATACCGGTCGAATTACTGTGTATCCTTATCTCCTTGATGTACAAAGTGACATTATTGG
AATGCGCAATACCCGGGTCGTGATCCCACTATTCCCGCTGAGAAATTACAAAGGTCCGCGCGCCGATCGTTTAACACCAG
TTGTCACCGTAGAAGGGGAAGAATACGTCGTGATGACTCACGAACTCGCCAGCATCCCACAACGCGTTCTGGGGGAAGAG
GTTTGTCATCTCAATCACCAACGTGAAGTCGTTAAAGCATCAATAGATTTTCTTTATGACGGGATTTAA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8898.08 Da
• Isoelectric Point: 5.6579

>AT120946 WP_004869059.1 NZ_CP038281:c2316829-2316593 [Raoultella ornithinolytica]
MPAVKHNKQNVSVTLDPILVKQAREIGINLSQTLTSALKSEIHSIEMARWQKENARAIDYLNDLTEENGLLSDEYRTF

Nucleotide

• Length: 237 bp

>AT120946 NZ_CP038281:c2316829-2316593 [Raoultella ornithinolytica]
ATGCCAGCCGTAAAACACAATAAGCAGAATGTCAGCGTGACGCTGGATCCTATACTGGTCAAACAGGCTCGTGAGATCGG
CATCAATCTTTCTCAAACGTTGACGAGCGCTCTAAAAAGCGAAATCCACAGCATCGAAATGGCACGCTGGCAAAAAGAAA
ATGCCAGGGCAATCGACTACCTCAATGATTTAACAGAGGAAAATGGACTTCTCTCTGACGAATACAGGACGTTTTAA

Structures

Antitoxin

• AlphaFold DB: A0A808XYT1