Detailed information of TA system TA120945

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 1596661..1597318
• Replicon: chromosome
• Accession: NZ_CP038281
• Organism: Raoultella ornithinolytica strain WLK218

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: -
• Locus tag: E4K08_RS11110
• Protein ID: WP_137056126.1
• Coordinates: 1596661..1597071 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: A0A514ELX1
• Locus tag: E4K08_RS11115
• Protein ID: WP_004867358.1
• Coordinates: 1597052..1597318 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K08_RS11090	1592583..1594316	-	1734	WP_137056125.1	single-stranded-DNA-specific exonuclease RecJ	-
E4K08_RS11095	1594322..1595035	-	714	WP_004867349.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
E4K08_RS11100	1595058..1595954	-	897	WP_032718225.1	site-specific tyrosine recombinase XerD	-
E4K08_RS11105	1596132..1596653	+	522	WP_004867353.1	flavodoxin FldB	-
E4K08_RS11110	1596661..1597071	-	411	WP_137056126.1	protein YgfX	Toxin
E4K08_RS11115	1597052..1597318	-	267	WP_004867358.1	FAD assembly factor SdhE	Antitoxin
E4K08_RS11120	1597615..1598598	+	984	WP_004867359.1	tRNA-modifying protein YgfZ	-
E4K08_RS11125	1598719..1599378	-	660	WP_004867362.1	hemolysin III family protein	-
E4K08_RS11130	1599540..1599851	-	312	WP_004867365.1	N(4)-acetylcytidine aminohydrolase	-
E4K08_RS11135	1599901..1600629	+	729	WP_004867368.1	MurR/RpiR family transcriptional regulator	-
E4K08_RS11140	1600749..1602182	+	1434	WP_004867372.1	6-phospho-beta-glucosidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-132)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 16207.11 Da
• Isoelectric Point: 11.0688

>T120945 WP_137056126.1 NZ_CP038281:c1597071-1596661 [Raoultella ornithinolytica]
VVLWQSDLRISWRSQWFSLLLHGIVAAIVLLMPWPLSYTPLWLLLLSLVVFDSVRSQRRIHACRGEIKLMTDSRLRWQKA
EWEIVGTPWVINSGMLLHLQDMQTRRRQHLWIAADSMDAKEWRDLRRLVLQKPAQE

Nucleotide

• Length: 411 bp

>T120945 NZ_CP038281:c1597071-1596661 [Raoultella ornithinolytica]
GTGGTCCTGTGGCAATCTGATTTACGCATCTCCTGGCGCTCACAGTGGTTCTCGCTGCTGCTGCACGGCATTGTGGCGGC
TATTGTGCTGCTGATGCCCTGGCCGCTGAGCTACACGCCGCTATGGCTATTGCTGCTGTCGCTGGTGGTGTTCGACAGCG
TGCGCAGCCAGCGGCGGATCCACGCCTGTCGGGGGGAAATAAAGCTGATGACCGATTCGCGTTTGCGTTGGCAAAAAGCG
GAATGGGAGATTGTCGGCACGCCGTGGGTGATTAATAGCGGGATGTTGCTGCATCTGCAGGATATGCAAACGCGTCGCCG
CCAGCATCTGTGGATCGCTGCAGACAGTATGGACGCCAAAGAGTGGCGAGATCTGCGCCGGTTGGTGTTACAAAAACCGG
CACAGGAGTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10553.05 Da
• Isoelectric Point: 4.8432

>AT120945 WP_004867358.1 NZ_CP038281:c1597318-1597052 [Raoultella ornithinolytica]
MDITNKARIHWACRRGMRELDISIMPFFEYEYDTLSDEDKQLFIRLLENDDPDLFNWLMNHGEPADAQLQRMVKLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT120945 NZ_CP038281:c1597318-1597052 [Raoultella ornithinolytica]
ATGGACATTACGAATAAAGCCCGCATCCACTGGGCATGCCGCAGGGGTATGCGTGAACTTGATATCTCCATCATGCCGTT
TTTCGAGTATGAGTACGATACGCTCAGCGATGAAGACAAACAGCTGTTTATCCGTCTGTTAGAAAACGACGATCCGGATC
TGTTTAACTGGCTGATGAATCATGGCGAACCGGCTGATGCGCAACTGCAACGGATGGTAAAATTAATCCAGACTCGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A514ELX1