120935

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 14236..14761 Replicon plasmid pWLK-KPC
Accession NZ_CP038279
Organism Raoultella ornithinolytica strain WLK218

Toxin (Protein)


Gene name ccdB Uniprot ID A0A6S4YF27
Locus tag E4K08_RS02955 Protein ID WP_025714517.1
Coordinates 14456..14761 (+) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID W8V2V6
Locus tag E4K08_RS02950 Protein ID WP_001568025.1
Coordinates 14236..14454 (+) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4K08_RS02925 9941..10501 + 561 WP_137055861.1 recombinase family protein -
E4K08_RS02930 10505..13471 + 2967 WP_001138014.1 Tn3-like element TnAs1 family transposase -
E4K08_RS02935 13468..13650 - 183 WP_137055862.1 hypothetical protein -
E4K08_RS02950 14236..14454 + 219 WP_001568025.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
E4K08_RS02955 14456..14761 + 306 WP_025714517.1 type II toxin-antitoxin system toxin CcdB Toxin
E4K08_RS02960 14930..15325 + 396 WP_017899885.1 hypothetical protein -
E4K08_RS02965 15352..15666 + 315 WP_053389906.1 hypothetical protein -
E4K08_RS02970 15677..16693 + 1017 WP_017899884.1 hypothetical protein -
E4K08_RS02975 16891..17685 + 795 WP_004197635.1 site-specific integrase -
E4K08_RS30575 18189..18491 - 303 WP_071571079.1 hypothetical protein -
E4K08_RS02985 18488..19114 - 627 WP_004197649.1 ParA family plasmid-partitioning AAA ATPase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaKPC-2 - 1..35262 35262


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11562.25 Da        Isoelectric Point: 5.6906

>T120935 WP_025714517.1 NZ_CP038279:14456-14761 [Raoultella ornithinolytica]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSCELYPVVHIGDESYRLMTTDMASVTSSVTGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T120935 NZ_CP038279:14456-14761 [Raoultella ornithinolytica]
ATGCAGTTTAAGGTTTATGCCTATAAAAGAGAGAGCCGCTATCGTCTGTTCGTGGATGTGCAGAGTGACATCATCGATAC
CCCCGGGCGACGGATGGTGATCCCGCTGGCCAGCGCCCGGCTGCTGTCGGATAAGGTTTCCTGTGAACTCTATCCGGTGG
TGCACATCGGGGATGAAAGCTACCGCCTGATGACCACGGACATGGCCAGCGTCACGTCCTCCGTCACCGGGGAGGAAGTG
GCCGATCTCAGCCACCGGGAAAATGACATCAAAAATGCGATAAACCTGATGTTCTGGGGAATTTGA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8392.34 Da        Isoelectric Point: 4.7405

>AT120935 WP_001568025.1 NZ_CP038279:14236-14454 [Raoultella ornithinolytica]
MKQRITVTIDSDSYQLLKSADVNISALVNRAMQNEARRLRSERWQAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT120935 NZ_CP038279:14236-14454 [Raoultella ornithinolytica]
ATGAAGCAGCGCATCACTGTCACCATAGACAGCGACAGTTATCAGTTACTGAAATCCGCAGACGTCAATATCTCCGCCCT
GGTGAACAGGGCCATGCAGAATGAGGCCCGTCGTCTGCGTTCCGAACGCTGGCAGGCTGAAAATCAGGAAGGGATGGCCG
AGGTTGCCCGCTTTATTGAGATGAACGGTTCATTCGCCGACGAAAACAGGGACTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6S4YF27

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4ZZP8

References