Detailed information of TA system TA120934

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 156830..157352
• Replicon: plasmid pWLK-238550
• Accession: NZ_CP038277
• Organism: Raoultella ornithinolytica strain WLK218

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: E4K08_RS02140
• Protein ID: WP_137055856.1
• Coordinates: 156830..157114 (-)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A7H0EVH5
• Locus tag: E4K08_RS02145
• Protein ID: WP_014839900.1
• Coordinates: 157104..157352 (-)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K08_RS02105	152086..152295	+	210	WP_125316032.1	hypothetical protein	-
E4K08_RS02110	152358..153302	+	945	WP_014839903.1	DsbA family protein	-
E4K08_RS02115	153383..153904	+	522	WP_048336588.1	hypothetical protein	-
E4K08_RS02120	153901..154503	+	603	WP_014839902.1	hypothetical protein	-
E4K08_RS30560	154744..155463	+	720	WP_042934502.1	cation:proton antiporter	-
E4K08_RS02130	155480..156157	-	678	WP_042934501.1	hypothetical protein	-
E4K08_RS02135	156163..156639	-	477	WP_042934526.1	hypothetical protein	-
E4K08_RS02140	156830..157114	-	285	WP_137055856.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4K08_RS02145	157104..157352	-	249	WP_014839900.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E4K08_RS02155	157693..159096	+	1404	WP_001567368.1	ISNCY-like element ISKpn21 family transposase	-
E4K08_RS02160	159125..159757	-	633	WP_001567369.1	hypothetical protein	-
E4K08_RS02165	160018..160902	-	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(6')-Ib-cr / blaOXA-1 / catB3 / ARR-3 / qacE / sul1 / armA / mph(E) / msr(E) / aadA5 / dfrA1	-	1..238550	238550

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-83)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10806.42 Da
• Isoelectric Point: 10.5526

>T120934 WP_137055856.1 NZ_CP038277:c157114-156830 [Raoultella ornithinolytica]
MTYKLAFNESALKEWKKLGHTIREQFKKKLSERLQNPRVPAAQLHGGKDQYKIKLHGAGYRLVYSVNDEIVTVTVIGVGK
RNNDDICNATRHRN

Nucleotide

• Length: 285 bp

>T120934 NZ_CP038277:c157114-156830 [Raoultella ornithinolytica]
ATGACCTATAAGTTAGCCTTCAATGAATCAGCTTTGAAAGAATGGAAAAAACTGGGTCATACTATTCGGGAGCAATTCAA
AAAGAAGCTTTCCGAACGGCTTCAGAACCCACGGGTGCCCGCAGCACAACTCCATGGAGGTAAAGACCAGTATAAAATAA
AGCTCCATGGCGCGGGTTACCGACTGGTTTACAGTGTCAACGATGAAATTGTGACGGTCACCGTAATTGGGGTGGGCAAA
CGCAACAATGATGATATCTGCAACGCAACCCGACACAGAAATTAA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9071.29 Da
• Isoelectric Point: 3.8832

>AT120934 WP_014839900.1 NZ_CP038277:c157352-157104 [Raoultella ornithinolytica]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMEMLEDQELGRIADERIGEDRIEVNID
DL

Nucleotide

• Length: 249 bp

>AT120934 NZ_CP038277:c157352-157104 [Raoultella ornithinolytica]
ATGGCATTTCAGGTTTTAACCACCACAGCAGCCAGCATTACAGAACTTAAACGTGACCCTATGGGTACCTTCAATGCAGG
AGAAGGTGCACCGGTAGCAATCCTGAATCGCAACGAACCCGCTTTTTACTGTGTACCACCAGCCCTGTATGCTCAGCTGA
TGGAAATGCTGGAAGATCAAGAGTTGGGGCGTATTGCTGATGAACGAATCGGTGAGGACCGAATCGAGGTCAACATTGAT
GACCTATAA

Structures

Antitoxin

• AlphaFold DB: A0A7H0EVH5