Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) tacAT/ElaA-DUF1778
Location 10579..11318 Replicon plasmid pWLK-107717
Accession NZ_CP038276
Organism Raoultella ornithinolytica strain WLK218

Toxin (Protein)


Gene name tacT Uniprot ID -
Locus tag E4K08_RS00725 Protein ID WP_076752153.1
Coordinates 10579..11064 (-) Length 162 a.a.

Antitoxin (Protein)


Gene name tacA Uniprot ID A0A5E1AWX8
Locus tag E4K08_RS00730 Protein ID WP_012540086.1
Coordinates 11052..11318 (-) Length 89 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4K08_RS00685 5769..5999 - 231 WP_076752138.1 hypothetical protein -
E4K08_RS00690 6088..6435 - 348 WP_076752140.1 hypothetical protein -
E4K08_RS00695 6538..6810 - 273 WP_137055850.1 hypothetical protein -
E4K08_RS00700 7700..8116 - 417 WP_076752145.1 helix-turn-helix domain-containing protein -
E4K08_RS00705 8113..8424 - 312 WP_076752146.1 type II toxin-antitoxin system HigB family toxin -
E4K08_RS00710 8574..8786 - 213 WP_076752148.1 hypothetical protein -
E4K08_RS00715 8894..9220 - 327 WP_076752150.1 hypothetical protein -
E4K08_RS00720 10079..10237 - 159 WP_076752152.1 type I toxin-antitoxin system Hok family toxin -
E4K08_RS00725 10579..11064 - 486 WP_076752153.1 GNAT family N-acetyltransferase Toxin
E4K08_RS00730 11052..11318 - 267 WP_012540086.1 DUF1778 domain-containing protein Antitoxin
E4K08_RS00735 11619..11942 + 324 WP_013087171.1 metalloregulator ArsR/SmtB family transcription factor -
E4K08_RS00740 11944..12657 + 714 WP_013087170.1 arsenical resistance protein ArsH -
E4K08_RS00745 12666..13211 + 546 WP_047365773.1 sigma-70 family RNA polymerase sigma factor -
E4K08_RS00750 13287..13649 + 363 WP_013087168.1 arsenic metallochaperone ArsD family protein -
E4K08_RS00755 13676..15427 + 1752 WP_175406650.1 arsenical pump-driving ATPase -
E4K08_RS00760 15552..16088 + 537 WP_013087166.1 GNAT family N-acetyltransferase -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..107717 107717


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(27-152)

Antitoxin

(2-87)


Sequences


Toxin        


Download         Length: 162 a.a.        Molecular weight: 17838.68 Da        Isoelectric Point: 10.0748

>T120933 WP_076752153.1 NZ_CP038276:c11064-10579 [Raoultella ornithinolytica]
VGRVTVPEPLSSFHQVAEFVCGETVLDDWLKQKGLKNQALGSARTFVVCKKDTKQVAGFYSLATGSVNHTEATGNLRRNM
PDPIPVIILARLAVDLSFRGKGLGADLLHDAVLRCYRVAENIGVRAIMVHALTEEAKNFYIHHGFKPSRTQQRTLFLKLP
Q

Download         Length: 486 bp

>T120933 NZ_CP038276:c11064-10579 [Raoultella ornithinolytica]
GTGGGACGTGTAACAGTACCAGAACCTTTATCCAGCTTTCATCAGGTGGCGGAATTCGTTTGCGGTGAAACTGTTCTGGA
TGACTGGTTAAAGCAGAAAGGCCTCAAAAACCAGGCTCTCGGATCGGCCAGAACGTTTGTAGTGTGTAAAAAAGACACGA
AACAAGTAGCCGGTTTTTACTCTCTGGCCACCGGGAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCCGATCCCATCCCTGTTATTATACTTGCCCGTCTTGCTGTCGATCTCTCTTTCCGCGGAAAAGGGCTTGGCGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGTGTACGTGCAATCATGGTTCATGCACTCACCG
AAGAAGCTAAAAATTTCTACATTCACCATGGTTTCAAACCATCACGAACTCAGCAACGGACATTATTTCTTAAACTCCCT
CAATAG

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10076.47 Da        Isoelectric Point: 4.3721

>AT120933 WP_012540086.1 NZ_CP038276:c11318-11052 [Raoultella ornithinolytica]
MKSDVQLNLRAKESQRALIDAAAEILHKSRTDFILEMACQAAENVILDRRVFNFNDEQYAEFIDMLDAPVADDPAIDKLL
ARKPQWDV

Download         Length: 267 bp

>AT120933 NZ_CP038276:c11318-11052 [Raoultella ornithinolytica]
ATGAAATCAGATGTTCAACTTAATCTTAGAGCAAAGGAATCACAGCGGGCGCTCATTGATGCTGCTGCGGAAATTCTTCA
CAAATCACGAACAGACTTCATCCTGGAAATGGCCTGTCAGGCCGCTGAGAACGTGATCCTCGATCGCCGGGTCTTTAATT
TTAACGATGAACAATATGCCGAATTCATCGACATGCTTGATGCCCCTGTTGCAGATGATCCTGCCATAGATAAACTGCTG
GCAAGGAAACCTCAGTGGGACGTGTAA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure


Antitoxin

Source ID Structure
AlphaFold DB A0A5E1AWX8

References