Detailed information of TA system TA120930

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2846734..2847332
• Replicon: chromosome
• Accession: NZ_CP038273
• Organism: Legionella israelensis strain Bercovier 4

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: E4T55_RS13125
• Protein ID: WP_058500963.1
• Coordinates: 2847135..2847332 (-)
• Length: 66 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: A0A0W0WHI8
• Locus tag: E4T55_RS13120
• Protein ID: WP_058500964.1
• Coordinates: 2846734..2847138 (-)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4T55_RS13085	2842007..2842795	-	789	WP_058500972.1	PhzF family phenazine biosynthesis protein	-
E4T55_RS13090	2842879..2843289	+	411	WP_058500971.1	MarR family transcriptional regulator	-
E4T55_RS13095	2843628..2844404	+	777	WP_058500970.1	integrase family protein	-
E4T55_RS13105	2844801..2845401	+	601	Protein_2571	IS6 family transposase	-
E4T55_RS15345	2845404..2845706	+	303	WP_058500967.1	helix-turn-helix domain-containing protein	-
E4T55_RS13115	2845710..2846306	+	597	WP_058500966.1	ImmA/IrrE family metallo-endopeptidase	-
E4T55_RS13120	2846734..2847138	-	405	WP_058500964.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
E4T55_RS13125	2847135..2847332	-	198	WP_058500963.1	type II toxin-antitoxin system HicA family toxin	Toxin
E4T55_RS13130	2847683..2848081	+	399	WP_131780761.1	hypothetical protein	-
E4T55_RS13135	2848132..2848455	+	324	WP_058500961.1	transposase	-
E4T55_RS13140	2848509..2849909	-	1401	WP_058500960.1	glycosyltransferase	-
E4T55_RS13145	2849909..2851186	-	1278	WP_058500959.1	nucleotide sugar dehydrogenase	-
E4T55_RS13150	2851322..2852284	-	963	WP_058500958.1	GDP-mannose 4,6-dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2841722..2862185	20463

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-126)

Sequences

Toxin

Protein

• Length: 66 a.a.
• Molecular weight: 7346.74 Da
• Isoelectric Point: 10.7302

>T120930 WP_058500963.1 NZ_CP038273:c2847332-2847135 [Legionella israelensis]
MKSADIIKIIERDGWVKCHQKGSHCQFKHPVKKGRVTVPHPKKDLPVKTVASIFKQAGISKEDIK

Nucleotide

• Length: 198 bp

>T120930 NZ_CP038273:c2847332-2847135 [Legionella israelensis]
ATGAAAAGCGCAGACATAATAAAAATAATTGAGCGTGACGGCTGGGTTAAATGCCATCAAAAAGGCAGCCATTGTCAGTT
CAAACACCCAGTAAAAAAAGGAAGGGTAACAGTACCTCATCCTAAAAAAGATTTGCCTGTTAAAACAGTTGCCAGCATTT
TTAAGCAGGCTGGTATTAGTAAGGAGGATATAAAATGA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 15003.97 Da
• Isoelectric Point: 4.4394

>AT120930 WP_058500964.1 NZ_CP038273:c2847138-2846734 [Legionella israelensis]
MRYPVVIHKDENSDFGVMAPDIPGCYSAGSTYDEALNNMVEAIECHLEGLLLDNETIPVGSSMDLWVHDEEFKEGVWAFV
DIDLSQISGKAKRINITIPERVLNLIDLYAKSHSIKNRSSFLADAALSYMESHK

Nucleotide

• Length: 405 bp

>AT120930 NZ_CP038273:c2847138-2846734 [Legionella israelensis]
ATGAGATATCCAGTTGTTATACATAAAGACGAAAACAGCGATTTTGGTGTTATGGCTCCAGATATCCCTGGCTGTTATTC
AGCAGGAAGTACTTATGATGAAGCGCTGAATAATATGGTAGAGGCTATTGAATGTCATCTTGAGGGATTACTATTGGATA
ATGAGACAATTCCAGTAGGATCATCAATGGACCTTTGGGTTCATGATGAAGAATTTAAAGAGGGCGTATGGGCATTTGTA
GATATAGATTTAAGTCAGATCTCAGGTAAAGCAAAACGTATAAATATTACTATACCGGAGCGAGTGTTAAATCTTATTGA
TCTCTACGCCAAAAGTCACTCGATAAAAAACCGGTCTTCTTTTCTTGCTGACGCCGCTCTTAGTTATATGGAAAGTCATA
AATAA

Structures

Antitoxin

• AlphaFold DB: A0A0W0WHI8