Detailed information of TA system TA120924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /Xre(antitoxin)
• Location: 43605..44647
• Replicon: chromosome
• Accession: NZ_CP038271
• Organism: Legionella geestiana strain 1308

Toxin (Protein)

• Gene name: -
• Uniprot ID: A0A0W0U467
• Locus tag: E4T54_RS00190
• Protein ID: WP_028387170.1
• Coordinates: 43605..44339 (-)
• Length: 245 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: E4T54_RS00195
• Protein ID: WP_081776818.1
• Coordinates: 44336..44647 (-)
• Length: 104 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4T54_RS00175	39168..42131	+	2964	WP_051551028.1	mechanosensitive ion channel	-
E4T54_RS00180	42088..42768	+	681	WP_028387168.1	DNA mismatch repair endonuclease MutH	-
E4T54_RS00185	43119..43511	-	393	WP_115152876.1	hypothetical protein	-
E4T54_RS00190	43605..44339	-	735	WP_028387170.1	RES family NAD+ phosphorylase	Toxin
E4T54_RS00195	44336..44647	-	312	WP_081776818.1	DUF2384 domain-containing protein	Antitoxin
E4T54_RS11960	44854..46896	-	2043	WP_028387172.1	hypothetical protein	-
E4T54_RS00200	47407..48186	-	780	WP_028387173.1	SDR family NAD(P)-dependent oxidoreductase	-
E4T54_RS00205	48173..49447	-	1275	WP_028387174.1	serine/threonine protein kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (59-194)

Antitoxin

• Antitoxin Xre/MbcA/ParS-like, toxin-binding domain (53-101)

Sequences

Toxin

Protein

• Length: 245 a.a.
• Molecular weight: 27775.13 Da
• Isoelectric Point: 5.3524

>T120924 WP_028387170.1 NZ_CP038271:c44339-43605 [Legionella geestiana]
VNLWQQLQGEAHIRPLSADPWRVVEAQHLSTTRFLVDSMEEHDLLEAMIESSKPAIEPSSDYLIYTPFRYPPLDYGSRFG
SAFEPSLWYGSLALKTAFAEVSWYRRLFLAQTSAELGYVETLLTAFQTRLETESGIHLSEPPFHEHREVISAPHTYATSQ
TLGSAMREAGVEVFSYYSARSEEPAINIAAFTSQVFRRKNGQFIRNQQTWQCIASKDTAEITRISLDGKQRYRFEGAVSF
ELGC

Nucleotide

• Length: 735 bp

>T120924 NZ_CP038271:c44339-43605 [Legionella geestiana]
GTGAACCTCTGGCAGCAGCTTCAGGGAGAAGCGCACATCCGCCCCTTGAGCGCAGACCCCTGGCGTGTGGTGGAAGCCCA
GCACCTTTCCACAACACGATTTCTCGTGGACAGCATGGAAGAGCATGACCTCCTTGAAGCCATGATTGAGTCCTCAAAAC
CCGCCATTGAGCCCAGCTCCGACTACCTTATTTACACGCCCTTTCGCTACCCGCCTCTAGATTATGGCTCGCGGTTTGGG
AGTGCATTTGAGCCCTCACTCTGGTATGGCTCACTTGCGCTTAAAACCGCTTTTGCCGAAGTGAGCTGGTACCGCCGACT
CTTTCTGGCACAAACCAGTGCTGAACTTGGCTATGTTGAAACGCTTCTCACTGCGTTTCAAACGCGCCTTGAAACAGAAA
GTGGCATTCATCTTTCAGAACCGCCCTTTCACGAACATCGCGAAGTAATTTCAGCGCCCCATACCTACGCAACCAGCCAG
ACACTGGGAAGCGCCATGCGCGAAGCTGGTGTTGAAGTCTTCAGCTATTATTCGGCACGCTCAGAAGAGCCCGCCATTAA
TATTGCCGCATTCACATCGCAGGTTTTTCGCAGAAAGAACGGTCAATTTATCAGAAACCAACAAACCTGGCAGTGCATTG
CCAGCAAAGATACGGCGGAAATCACCCGCATCAGCCTTGATGGAAAACAACGCTACCGTTTTGAGGGTGCAGTGTCTTTT
GAACTGGGGTGTTGA

Antitoxin

Protein

• Length: 104 a.a.
• Molecular weight: 11633.44 Da
• Isoelectric Point: 9.8579

>AT120924 WP_081776818.1 NZ_CP038271:c44647-44336 [Legionella geestiana]
MCTHYNLSARELSEILGVSEASTTRMHQGKKWISPDSKEGEIALLLLRVYRSLNALVGNHNEKAQSWLRAQNHYLRCTPV
NALKTVTGLVEVVRYLDALRGKL

Nucleotide

• Length: 312 bp

>AT120924 NZ_CP038271:c44647-44336 [Legionella geestiana]
ATGTGTACCCATTATAACCTGAGTGCGCGTGAGCTTTCGGAAATCCTTGGGGTCAGTGAGGCCAGTACCACCCGCATGCA
CCAGGGGAAAAAATGGATTAGCCCGGATTCTAAAGAAGGTGAAATTGCCCTGCTGCTCCTGCGGGTGTATCGAAGCCTCA
ATGCGCTTGTTGGTAATCACAATGAAAAAGCACAAAGCTGGCTGCGTGCGCAAAACCATTACCTGCGCTGCACACCGGTT
AATGCCTTAAAAACTGTGACGGGGCTTGTGGAGGTGGTGCGCTACCTCGATGCTCTGCGGGGGAAACTGTGA

Structures

Toxin

• AlphaFold DB: A0A0W0U467