Detailed information of TA system TA120920

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2109958..2110487
• Replicon: chromosome
• Accession: NZ_CP038270
• Organism: Staphylococcus aureus strain O408 isolate B115

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: SaO408_RS10820
• Protein ID: WP_000621175.1
• Coordinates: 2109958..2110320 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: SaO408_RS10825
• Protein ID: WP_000948331.1
• Coordinates: 2110317..2110487 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SaO408_RS10795	2106937..2107707	-	771	WP_001041107.1	RNA polymerase sigma factor SigB	-
SaO408_RS10800	2107682..2108161	-	480	WP_001190825.1	anti-sigma B factor RsbW	-
SaO408_RS10805	2108163..2108489	-	327	WP_001052491.1	anti-sigma factor antagonist	-
SaO408_RS10810	2108608..2109609	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
SaO408_RS10820	2109958..2110320	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
SaO408_RS10825	2110317..2110487	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
SaO408_RS10830	2110572..2111720	-	1149	WP_001281146.1	alanine racemase	-
SaO408_RS10835	2111786..2112145	-	360	WP_000581198.1	holo-ACP synthase	-
SaO408_RS10840	2112149..2112640	-	492	WP_001205919.1	PH domain-containing protein	-
SaO408_RS10845	2112627..2114210	-	1584	WP_001294641.1	PH domain-containing protein	-
SaO408_RS10850	2114203..2114682	-	480	WP_001287083.1	hypothetical protein	-
SaO408_RS10855	2114891..2115451	-	561	WP_001092415.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T120920 WP_000621175.1 NZ_CP038270:c2110320-2109958 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T120920 NZ_CP038270:c2110320-2109958 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGATTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCATCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT120920 WP_000948331.1 NZ_CP038270:c2110487-2110317 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT120920 NZ_CP038270:c2110487-2110317 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCTATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1