Detailed information of TA system TA120886

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 108469..109057
• Replicon: plasmid pEC_gr13
• Accession: NZ_CP038263
• Organism: Acinetobacter baumannii strain LEV1449/17Ec

Toxin (Protein)

• Gene name: relE
• Uniprot ID: A0A5K1MH32
• Locus tag: E4K03_RS18670
• Protein ID: WP_000569859.1
• Coordinates: 108469..108765 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E4K03_RS18675
• Protein ID: WP_000241337.1
• Coordinates: 108767..109057 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K03_RS18625	103559..104071	-	513	WP_001089574.1	SMI1/KNR4 family protein	-
E4K03_RS18630	104110..104628	-	519	WP_000447195.1	sel1 repeat family protein	-
E4K03_RS18635	104803..105012	-	210	WP_000069475.1	hypothetical protein	-
E4K03_RS18640	105005..105307	-	303	WP_001129974.1	XRE family transcriptional regulator	-
E4K03_RS18645	105300..105656	-	357	WP_000269904.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4K03_RS18650	106184..106501	-	318	WP_000900860.1	hypothetical protein	-
E4K03_RS18660	106813..108138	+	1326	WP_008942541.1	ISNCY family transposase	-
E4K03_RS18670	108469..108765	+	297	WP_000569859.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4K03_RS18675	108767..109057	+	291	WP_000241337.1	putative addiction module antidote protein	Antitoxin
E4K03_RS18680	109356..109508	-	153	Protein_101	transposase	-
E4K03_RS18685	109575..109736	+	162	Protein_102	transposase	-
E4K03_RS18690	109788..111006	+	1219	Protein_103	IS3 family transposase	-
E4K03_RS18695	111243..111575	+	333	WP_001128380.1	zinc ribbon domain-containing protein	-
E4K03_RS18700	111602..111724	-	123	WP_109267661.1	helix-turn-helix domain-containing protein	-
E4K03_RS18705	111975..112250	+	276	WP_001133596.1	metal/formaldehyde-sensitive transcriptional repressor	-
E4K03_RS18710	112272..113384	+	1113	WP_000842149.1	S-(hydroxymethyl)glutathione dehydrogenase/class III alcohol dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..128013	128013
	-	inside	IScluster/Tn	-	-	100426..111006	10580

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-89)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-92)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11554.35 Da
• Isoelectric Point: 8.5037

>T120886 WP_000569859.1 NZ_CP038263:108469-108765 [Acinetobacter baumannii]
MIEIKRLPEFDEWLDGLKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGAGWRMYYIQHGDVVIVMLGGGEKS
TQKDDIKKAVKLSKTLED

Nucleotide

• Length: 297 bp

>T120886 NZ_CP038263:108469-108765 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTGCCTGAATTTGATGAATGGCTAGATGGTCTTAAAGACAATATGACTCGAATCCGCTTGAA
TCGTCGATTAGATAAGGTCCAACGTGGTAATTGGGGTGACATTAAACCACTCCAAGATGGTGTCTGGGAAATGAGAGAGT
TCTTTGGTGCTGGTTGGCGGATGTACTACATCCAACATGGTGACGTTGTGATCGTGATGCTTGGTGGTGGTGAAAAATCT
ACACAGAAAGATGACATCAAGAAAGCGGTCAAACTATCGAAAACATTGGAGGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10584.24 Da
• Isoelectric Point: 5.1133

>AT120886 WP_000241337.1 NZ_CP038263:108767-109057 [Acinetobacter baumannii]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEENDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRQDSTPRFDTI
NRVVNALGLKLTVQHT

Nucleotide

• Length: 291 bp

>AT120886 NZ_CP038263:108767-109057 [Acinetobacter baumannii]
ATGGTTAAAGTCGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGATATTGTAATGTACCTCAA
TATGGTTCTTGAAGAAAATGATCCTGCTGAATTAGCGCACGCACTTGGTGTAATTGCTAAAGCAAGAGGTATGACCCAAA
TTGCGAAAGAAGCAGGTATTGGACGAGAAGCCCTTTACAAAGCACTTCGTCAAGACTCAACGCCTCGTTTTGATACGATC
AACCGTGTTGTGAATGCTTTAGGTTTAAAATTGACAGTTCAACATACTTAA

Structures

Toxin

• AlphaFold DB: A0A5K1MH32