Detailed information of TA system TA120878

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/upstrm_HI1419-dnstrm_HI1420
• Location: 103386..103974
• Replicon: plasmid pEH_gr13
• Accession: NZ_CP038259
• Organism: Acinetobacter baumannii strain 39741

Toxin (Protein)

• Gene name: relE
• Uniprot ID: N9CKJ9
• Locus tag: E4K02_RS19030
• Protein ID: WP_004647749.1
• Coordinates: 103386..103682 (+)
• Length: 99 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: E4K02_RS19035
• Protein ID: WP_031953691.1
• Coordinates: 103684..103974 (+)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K02_RS18995	98650..99273	-	624	Protein_105	GIY-YIG nuclease family protein	-
E4K02_RS19000	99364..99872	+	509	Protein_106	transposase	-
E4K02_RS19005	99940..101105	+	1166	WP_085940844.1	IS3-like element ISAba22 family transposase	-
E4K02_RS19010	101168..101806	+	639	WP_002009048.1	IS3 family transposase	-
E4K02_RS19015	102058..102360	+	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4K02_RS19020	102361..102642	+	282	WP_000985610.1	putative addiction module antidote protein	-
E4K02_RS19025	102750..103277	+	528	WP_002124964.1	plasmid pRiA4b ORF-3 family protein	-
E4K02_RS19030	103386..103682	+	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4K02_RS19035	103684..103974	+	291	WP_031953691.1	putative addiction module antidote protein	Antitoxin
E4K02_RS19040	104070..104330	-	261	WP_079377198.1	AraC family transcriptional regulator	-
E4K02_RS19045	104595..105290	+	696	Protein_115	IS256 family transposase	-
E4K02_RS19050	105247..105705	-	459	WP_031953687.1	hypothetical protein	-
E4K02_RS19060	105747..106679	+	933	WP_031953685.1	IS5-like element ISAba13 family transposase	-
E4K02_RS19065	106647..107101	-	455	Protein_118	hypothetical protein	-
E4K02_RS19710	107481..107582	-	102	Protein_119	DNA-binding protein	-
E4K02_RS19075	107574..107795	-	222	Protein_120	IS3 family transposase	-
E4K02_RS19735	107938..108222	-	285	Protein_121	antibiotic biosynthesis monooxygenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..135229	135229
	-	inside	IScluster/Tn	-	-	85108..106679	21571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (3-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 99 a.a.
• Molecular weight: 11584.25 Da
• Isoelectric Point: 5.8801

>T120878 WP_004647749.1 NZ_CP038259:103386-103682 [Acinetobacter baumannii]
MIEIKRLPEFDEWLDGIKDNMTRIRLNRRLDKVQRGNWGDIKPLQDGVWEMREFFGSGWRMYYIQHGDVVIVMLGGGDKS
TQQQDIDRAVKLSKTLED

Nucleotide

• Length: 297 bp

>T120878 NZ_CP038259:103386-103682 [Acinetobacter baumannii]
ATGATTGAAATTAAACGTCTACCAGAATTTGATGAATGGTTGGATGGCATTAAAGACAATATGACCCGCATTCGCTTGAA
TCGCCGATTAGATAAGGTTCAACGCGGGAACTGGGGAGACATTAAACCTCTCCAAGATGGTGTTTGGGAAATGAGAGAGT
TCTTCGGTTCAGGATGGAGGATGTATTACATCCAACATGGTGACGTAGTGATTGTAATGCTTGGTGGCGGAGATAAATCA
ACTCAACAACAAGACATCGATCGTGCAGTCAAATTATCGAAAACGTTGGAGGATTAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10574.26 Da
• Isoelectric Point: 6.2229

>AT120878 WP_031953691.1 NZ_CP038259:103684-103974 [Acinetobacter baumannii]
MVKVADLPSFDMAESLKTEEDIVMYLNMVLEDNDPAELAHALGVIAKARGMTQIAKEAGIGREALYKALRHDSAPRFDTI
NRVVNALGLKLRVQHA

Nucleotide

• Length: 291 bp

>AT120878 NZ_CP038259:103684-103974 [Acinetobacter baumannii]
ATGGTTAAAGTAGCTGATTTACCAAGTTTTGATATGGCTGAGTCACTCAAAACTGAAGAAGACATCGTGATGTATCTCAA
TATGGTTCTTGAAGATAATGATCCTGCTGAATTAGCTCATGCCCTTGGTGTCATTGCTAAAGCTAGAGGTATGACCCAAA
TTGCTAAAGAAGCAGGGATCGGACGTGAAGCACTTTATAAAGCACTTCGCCATGATTCAGCACCACGTTTTGATACGATT
AATCGTGTAGTAAATGCTTTAGGCTTAAAACTAAGAGTGCAACACGCCTAA