Detailed information of TA system TA120877

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): PumAB/upstrm_HI1419-dnstrm_HI1420
• Location: 102058..102642
• Replicon: plasmid pEH_gr13
• Accession: NZ_CP038259
• Organism: Acinetobacter baumannii strain 39741

Toxin (Protein)

• Gene name: PumA
• Uniprot ID: N9JY46
• Locus tag: E4K02_RS19015
• Protein ID: WP_000286963.1
• Coordinates: 102058..102360 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: PumB
• Uniprot ID: -
• Locus tag: E4K02_RS19020
• Protein ID: WP_000985610.1
• Coordinates: 102361..102642 (+)
• Length: 94 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4K02_RS18990	97570..98595	-	1026	WP_010326927.1	IS30 family transposase	-
E4K02_RS18995	98650..99273	-	624	Protein_105	GIY-YIG nuclease family protein	-
E4K02_RS19000	99364..99872	+	509	Protein_106	transposase	-
E4K02_RS19005	99940..101105	+	1166	WP_085940844.1	IS3-like element ISAba22 family transposase	-
E4K02_RS19010	101168..101806	+	639	WP_002009048.1	IS3 family transposase	-
E4K02_RS19015	102058..102360	+	303	WP_000286963.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
E4K02_RS19020	102361..102642	+	282	WP_000985610.1	putative addiction module antidote protein	Antitoxin
E4K02_RS19025	102750..103277	+	528	WP_002124964.1	plasmid pRiA4b ORF-3 family protein	-
E4K02_RS19030	103386..103682	+	297	WP_004647749.1	type II toxin-antitoxin system RelE/ParE family toxin	-
E4K02_RS19035	103684..103974	+	291	WP_031953691.1	putative addiction module antidote protein	-
E4K02_RS19040	104070..104330	-	261	WP_079377198.1	AraC family transcriptional regulator	-
E4K02_RS19045	104595..105290	+	696	Protein_115	IS256 family transposase	-
E4K02_RS19050	105247..105705	-	459	WP_031953687.1	hypothetical protein	-
E4K02_RS19060	105747..106679	+	933	WP_031953685.1	IS5-like element ISAba13 family transposase	-
E4K02_RS19065	106647..107101	-	455	Protein_118	hypothetical protein	-
E4K02_RS19710	107481..107582	-	102	Protein_119	DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..135229	135229
	-	inside	IScluster/Tn	-	-	85108..106679	21571

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Addiction module killer protein, predicted (1-97)

Antitoxin

• Cro/C1-type helix-turn-helix domain (47-91)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 11545.02 Da
• Isoelectric Point: 4.6838

>T120877 WP_000286963.1 NZ_CP038259:102058-102360 [Acinetobacter baumannii]
MYSIYTTEAFDDWFTKLKDQQAKRRIQVRIDRVEDGNFGDTEPVGEGVSELRFFFGPGYRIYYCKQGQRVVILLAGGDKS
TQSKDIKLALQLAQDLEEEL

Nucleotide

• Length: 303 bp

>T120877 NZ_CP038259:102058-102360 [Acinetobacter baumannii]
ATGTACTCAATATATACCACAGAAGCCTTTGATGATTGGTTTACCAAGTTAAAAGATCAACAGGCAAAAAGACGCATACA
AGTCCGAATTGATCGGGTTGAAGATGGGAACTTTGGAGATACTGAACCAGTCGGTGAAGGTGTTTCTGAGTTACGTTTCT
TCTTTGGACCTGGCTATAGGATTTATTACTGCAAGCAAGGGCAAAGGGTCGTTATTCTCTTAGCAGGCGGAGATAAGTCT
ACGCAAAGTAAAGATATAAAACTTGCCCTACAATTAGCACAAGATTTAGAGGAGGAGCTGTAA

Antitoxin

Protein

• Length: 94 a.a.
• Molecular weight: 10109.67 Da
• Isoelectric Point: 8.0018

>AT120877 WP_000985610.1 NZ_CP038259:102361-102642 [Acinetobacter baumannii]
MAIKLRKWDSAEHLKTEEDMQAYLQACIDESNGDAAFIAKALGNIAKAKGMAQLSRDTGLGRESLYKALSGDVNPSFDTV
IKVVKALNLRLAI

Nucleotide

• Length: 282 bp

>AT120877 NZ_CP038259:102361-102642 [Acinetobacter baumannii]
ATGGCTATCAAACTTCGTAAATGGGATAGTGCTGAGCATCTTAAAACAGAAGAAGATATGCAAGCTTACCTACAAGCCTG
TATCGATGAATCTAATGGTGATGCTGCATTCATTGCTAAAGCTTTAGGTAACATTGCTAAAGCTAAAGGAATGGCTCAAT
TATCACGAGATACAGGTCTAGGCCGAGAAAGTCTTTACAAAGCACTTTCTGGTGATGTTAACCCTAGTTTTGATACTGTG
ATTAAAGTTGTAAAAGCACTGAATTTACGCTTAGCAATTTAA

Structures

Toxin

• AlphaFold DB: A0A836MGN2