Detailed information of TA system TA120867

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 67343..68883
• Replicon: chromosome
• Accession: NZ_CP038254
• Organism: Legionella israelensis strain HL-0427-4011

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: -
• Locus tag: E3983_RS00330
• Protein ID: WP_135059452.1
• Coordinates: 67343..68650 (-)
• Length: 436 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0W0V2M6
• Locus tag: E3983_RS00335
• Protein ID: WP_058503011.1
• Coordinates: 68644..68883 (-)
• Length: 80 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3983_RS00310	62477..64033	+	1557	WP_135059450.1	hypothetical protein	-
E3983_RS00315	64290..65464	+	1175	Protein_63	ISL3 family transposase	-
E3983_RS00320	65489..65632	-	144	Protein_64	IS4 family transposase	-
E3983_RS00325	66052..67302	+	1251	WP_135059451.1	integrase family protein	-
E3983_RS00330	67343..68650	-	1308	WP_135059452.1	type II toxin-antitoxin system HipA family toxin	Toxin
E3983_RS00335	68644..68883	-	240	WP_058503011.1	helix-turn-helix domain-containing protein	Antitoxin
E3983_RS00340	69437..69709	+	273	WP_135059453.1	helix-turn-helix domain-containing protein	-
E3983_RS00345	69798..71549	+	1752	WP_135059454.1	DUF927 domain-containing protein	-
E3983_RS00350	71648..72457	-	810	WP_135059455.1	integrase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	64290..65189	899
	-	flank	IS/Tn	-	-	73513..74763	1250

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (150-400)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 436 a.a.
• Molecular weight: 49727.17 Da
• Isoelectric Point: 8.2161

>T120867 WP_135059452.1 NZ_CP038254:c68650-67343 [Legionella israelensis]
MVMRKLYILMNHELVGELTQDNAGGLSFSYVADWLSKPHSRPVSLSLPLITQKFSGEVVYNFFDNLLPDNPQIRARIQAR
FQASSSRPFDLLALIGSDCVGAIQIVEKIPERQEKKIECKIVSEAEIAHTLRSYQISPLGMDKEDDEFRISIAGAQEKTA
FLYYQNKWHKPFGPTPTTHIFKLPIGYLSHQGIDLSESCENEHLCLLLASKFGLDVAKSKILQFEDVKVLVVERFDRKWS
RDNRYILRLPQEDLCQSLGYSPNLKYQSDGGPGIKEIMQLLNGSQQPQKDRDEFFRAQVVYWCLGAIDAHAKNFSISIQA
QGGYQLTPLYDIISAYPLVEQKQLQFKKLKMAMALYGKNSHYKIDGIQKRHFIETAKYVQYSKIKAAQIFEQVIDRIESA
IDEVSTELPNHFPERIADPIFKGMRAFKARMTSTS

Nucleotide

• Length: 1308 bp

>T120867 NZ_CP038254:c68650-67343 [Legionella israelensis]
ATGGTAATGCGTAAATTATACATATTAATGAACCATGAGCTCGTTGGGGAGCTTACCCAGGATAATGCAGGAGGTTTAAG
TTTTTCCTACGTTGCTGACTGGCTTTCCAAGCCGCATTCACGGCCGGTTTCTTTATCGCTACCATTAATAACGCAAAAAT
TCTCAGGTGAAGTTGTTTATAACTTTTTTGATAACTTACTCCCTGATAATCCGCAGATAAGAGCCAGAATTCAAGCTCGT
TTTCAGGCAAGTTCATCACGACCGTTTGATTTATTAGCTTTAATTGGTAGTGATTGTGTTGGTGCAATACAAATCGTTGA
AAAAATTCCCGAGAGACAAGAAAAAAAAATTGAGTGTAAAATTGTTAGTGAGGCCGAGATAGCTCATACTTTACGTTCCT
ATCAGATCTCGCCACTGGGAATGGATAAAGAAGATGATGAATTTAGAATATCGATAGCAGGCGCTCAGGAAAAAACCGCT
TTTCTATATTATCAAAACAAATGGCATAAACCATTTGGACCTACACCAACAACTCATATATTCAAACTACCTATTGGGTA
TTTATCTCATCAAGGGATTGATTTAAGTGAGAGTTGTGAAAATGAACATCTCTGTTTACTATTGGCGAGCAAATTTGGTT
TAGATGTTGCGAAATCTAAAATCTTACAATTCGAAGATGTAAAAGTATTGGTTGTCGAAAGATTTGATAGAAAATGGAGT
CGAGATAATCGGTATATTCTCAGATTGCCGCAGGAAGATCTTTGCCAATCATTGGGATACTCACCAAATTTAAAATATCA
GTCTGATGGTGGGCCGGGTATTAAAGAAATTATGCAGCTACTTAATGGCTCACAGCAGCCTCAAAAAGATAGAGATGAAT
TTTTCAGGGCACAGGTTGTTTACTGGTGCCTTGGTGCTATAGATGCGCATGCCAAAAATTTCAGCATAAGCATACAAGCG
CAAGGCGGTTACCAGCTAACCCCATTATACGACATCATTTCAGCCTATCCTTTAGTCGAACAAAAACAGTTACAGTTTAA
GAAATTGAAAATGGCAATGGCGTTATATGGTAAAAACAGTCATTATAAGATTGACGGCATTCAAAAAAGACACTTTATTG
AAACGGCAAAATACGTTCAATATTCTAAAATTAAAGCAGCTCAGATTTTTGAACAAGTGATTGACCGTATCGAATCTGCT
ATTGATGAGGTCTCAACAGAGTTACCAAACCACTTCCCAGAAAGGATTGCAGATCCAATTTTCAAAGGGATGAGAGCTTT
TAAGGCACGAATGACGTCCACTTCATGA

Antitoxin

Protein

• Length: 80 a.a.
• Molecular weight: 9023.30 Da
• Isoelectric Point: 4.7672

>AT120867 WP_058503011.1 NZ_CP038254:c68883-68644 [Legionella israelensis]
MIISSAKDLALYLNDKRKQLKMSQSEVADLVGLKQDTISKFENSPDNSRIDTLFRILSALNLNMSLVEKEQAAHEEIEW

Nucleotide

• Length: 240 bp

>AT120867 NZ_CP038254:c68883-68644 [Legionella israelensis]
ATGATCATCAGCTCAGCAAAAGATTTAGCATTGTATTTGAATGACAAGCGTAAGCAACTCAAAATGAGTCAATCTGAAGT
TGCCGATTTAGTCGGTTTAAAACAGGATACCATATCAAAGTTTGAAAATAGCCCTGATAATAGCCGAATTGATACTTTAT
TTAGAATATTGTCTGCTCTTAATTTAAATATGAGTCTTGTTGAAAAAGAGCAAGCGGCTCATGAAGAGATAGAATGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A0W0V2M6