Detailed information of TA system TA120835

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2071633..2072162
• Replicon: chromosome
• Accession: NZ_CP038229
• Organism: Staphylococcus aureus strain MJ163

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E4J95_RS10120
• Protein ID: WP_000621175.1
• Coordinates: 2071633..2071995 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E4J95_RS10125
• Protein ID: WP_000948331.1
• Coordinates: 2071992..2072162 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E4J95_RS10100	2068611..2069381	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
E4J95_RS10105	2069356..2069835	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
E4J95_RS10110	2069837..2070163	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E4J95_RS10115	2070282..2071283	-	1002	WP_000390829.1	PP2C family protein-serine/threonine phosphatase	-
E4J95_RS10120	2071633..2071995	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E4J95_RS10125	2071992..2072162	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E4J95_RS10130	2072247..2073395	-	1149	WP_001281154.1	alanine racemase	-
E4J95_RS10135	2073461..2073820	-	360	WP_000581197.1	holo-ACP synthase	-
E4J95_RS10140	2073824..2074315	-	492	WP_072353916.1	PH domain-containing protein	-
E4J95_RS10145	2074302..2075885	-	1584	WP_045177766.1	PH domain-containing protein	-
E4J95_RS10150	2075878..2076357	-	480	WP_169449295.1	hypothetical protein	-
E4J95_RS10155	2076566..2077126	-	561	WP_001092409.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T120835 WP_000621175.1 NZ_CP038229:c2071995-2071633 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T120835 NZ_CP038229:c2071995-2071633 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAATTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT120835 WP_000948331.1 NZ_CP038229:c2072162-2071992 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT120835 NZ_CP038229:c2072162-2071992 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1