Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) phd-doc/HTH_IclR(antitoxin)
Location 1213357..1215119 Replicon chromosome
Accession NZ_CP038225
Organism Burkholderia pseudomallei strain Yap2a

Toxin (Protein)


Gene name doc Uniprot ID A3NIY6
Locus tag E4F35_RS26525 Protein ID WP_004531730.1
Coordinates 1214208..1215119 (-) Length 304 a.a.

Antitoxin (Protein)


Gene name phd Uniprot ID Q63LW2
Locus tag E4F35_RS26520 Protein ID WP_004195450.1
Coordinates 1213357..1214211 (-) Length 285 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4F35_RS26500 1208573..1210063 - 1491 WP_004524006.1 aldehyde dehydrogenase -
E4F35_RS26505 1210089..1210904 - 816 WP_150977257.1 aspartate dehydrogenase -
E4F35_RS26510 1210897..1212570 - 1674 WP_004524004.1 thiamine pyrophosphate-binding protein -
E4F35_RS26515 1212563..1213360 - 798 WP_004524003.1 SDR family oxidoreductase -
E4F35_RS26520 1213357..1214211 - 855 WP_004195450.1 IclR family transcriptional regulator Antitoxin
E4F35_RS26525 1214208..1215119 - 912 WP_004531730.1 alpha/beta fold hydrolase Toxin
E4F35_RS26530 1215136..1215663 - 528 WP_004524001.1 cupin domain-containing protein -
E4F35_RS26535 1215702..1216472 - 771 WP_004184755.1 SDR family oxidoreductase -
E4F35_RS26540 1216469..1217536 - 1068 WP_004195457.1 aromatic ring-hydroxylating dioxygenase subunit alpha -
E4F35_RS26545 1217571..1217882 - 312 WP_004184841.1 hypothetical protein -
E4F35_RS26550 1217884..1218201 - 318 WP_004184916.1 non-heme iron oxygenase ferredoxin subunit -
E4F35_RS26560 1218635..1219723 + 1089 WP_024428709.1 porin -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Alpha/beta hydrolase fold-1

(54-286)

Antitoxin

Transcription regulator IclR, C-terminal

(143-265)


Sequences

Toxin        


Download         Length: 304 a.a.        Molecular weight: 30712.87 Da        Isoelectric Point: 8.4944

>T120827 WP_004531730.1 NZ_CP038225:c1215119-1214208 [Burkholderia pseudomallei]
MSTISTTTTEAGAAPFAALLARFPERRAAVGGAGVIGYREAGEAHARRAGCEPVVLLHGIGSGAASWVRQLDALGATRRA
LAWDAPGYGASSRVAADSPVAADYAASLAAWLDALRIERCVLVGHSLGAIMAGAFARIAGERLGGLLLISPAGGYGSAPA
DVRAGRRDSRLALLASLGPQGLAAERSANMLSAQASGAAREWVRWNMARIAPAGYAQATHLLANADLASDLAHCRGRIAV
AVAVGGDDAITPPAACERIARAAGVGLQVIARAGHAGYVEAPDAYSALIDAFCRQCDGSRGTP

Download         Length: 912 bp

>T120827 NZ_CP038225:c1215119-1214208 [Burkholderia pseudomallei]
ATGAGCACGATTTCCACGACGACAACCGAGGCGGGCGCGGCGCCGTTCGCCGCGCTGCTCGCACGCTTTCCCGAGCGGCG
CGCGGCGGTGGGCGGCGCCGGCGTGATCGGCTATCGCGAGGCGGGCGAAGCCCATGCGCGGCGCGCCGGATGCGAACCCG
TCGTGCTGCTGCACGGGATCGGCTCGGGGGCGGCGTCGTGGGTGCGCCAGCTCGATGCGCTCGGCGCGACGCGTCGCGCG
CTCGCGTGGGACGCACCCGGCTATGGCGCGTCGTCGCGCGTCGCAGCCGATTCGCCCGTCGCGGCGGACTACGCGGCGTC
GCTCGCCGCGTGGCTCGACGCGCTGCGCATCGAGCGCTGCGTGCTCGTCGGCCATTCGCTCGGCGCGATCATGGCCGGCG
CGTTTGCACGGATCGCGGGCGAGCGGCTGGGGGGATTGCTGCTGATTTCCCCCGCGGGCGGCTACGGCAGCGCGCCCGCC
GACGTGCGGGCCGGGCGGCGCGACAGCCGCCTTGCGTTGCTCGCGTCGCTCGGCCCGCAAGGGCTCGCCGCCGAGCGCAG
CGCGAACATGCTGTCCGCGCAGGCGAGCGGCGCGGCGCGCGAGTGGGTGCGCTGGAACATGGCGCGCATCGCGCCCGCGG
GCTACGCGCAGGCGACGCATCTGCTCGCGAACGCGGATCTCGCCTCCGATCTTGCCCATTGCCGCGGCCGCATCGCGGTC
GCGGTCGCGGTCGGCGGCGACGATGCGATCACGCCGCCCGCCGCATGCGAGCGGATCGCGCGGGCGGCGGGCGTCGGGCT
GCAGGTGATCGCGCGCGCGGGGCACGCCGGCTACGTCGAAGCGCCCGATGCATACAGCGCGTTGATCGACGCCTTTTGCC
GTCAATGCGACGGATCGCGAGGCACACCATGA

Antitoxin


Download         Length: 285 a.a.        Molecular weight: 31152.56 Da        Isoelectric Point: 6.8932

>AT120827 WP_004195450.1 NZ_CP038225:c1214211-1213357 [Burkholderia pseudomallei]
MSEPLLKDADDARNDSNYVVPGLERGLRILAEFSPREPVLGAPELSKRLGIPRTTVFRLLQTLESLGFLERADKDRNYRL
GVAVLRLGFEYLSSLELTDLGLPVIESLRDATGFTAHIVIRDGRDVVFVAKAQNAGNAFGSVRVNVGTRLPAHATTHGHV
LMGDLSLAQLRALYPERALERVTKATPETVDALFDVIRDDARRGYGIGNSAFERDISVVTAPVRNEASRIVAVVTVTVPR
PEIDAALVAEGLVEKVVRAAAELSRRLNYRSDDENTYLKALGLR

Download         Length: 855 bp

>AT120827 NZ_CP038225:c1214211-1213357 [Burkholderia pseudomallei]
ATGAGCGAGCCGCTGCTGAAGGACGCCGACGACGCGAGGAATGATTCGAACTACGTCGTACCGGGGCTCGAGCGCGGGCT
GCGGATCCTGGCCGAGTTCTCGCCGCGAGAGCCGGTGCTCGGCGCGCCGGAGCTGTCGAAGCGGCTCGGCATTCCGCGCA
CGACGGTGTTCCGCCTGCTGCAGACGCTCGAATCGCTCGGCTTTCTCGAGCGCGCGGACAAGGATCGCAACTATCGCCTG
GGCGTCGCGGTGCTGCGCCTCGGCTTCGAATACCTGAGCTCGCTCGAGCTGACCGATCTCGGCTTGCCGGTGATCGAAAG
CCTGCGCGATGCGACGGGCTTCACCGCGCACATCGTGATTCGCGACGGCCGCGACGTCGTGTTCGTCGCGAAGGCGCAAA
ACGCGGGGAACGCGTTCGGCTCGGTGCGGGTGAACGTCGGCACGCGCCTGCCCGCGCATGCGACGACGCACGGCCACGTG
CTGATGGGCGACCTGTCGCTCGCGCAGTTGCGCGCGTTGTATCCGGAGCGGGCGCTCGAACGCGTGACGAAGGCGACGCC
CGAGACCGTCGACGCGCTCTTCGACGTGATCCGCGACGATGCGCGGCGCGGCTACGGCATCGGCAACTCGGCGTTCGAGC
GCGACATCTCGGTCGTCACGGCGCCCGTGCGCAACGAAGCGAGCCGGATCGTCGCGGTCGTCACGGTGACGGTGCCGCGG
CCGGAGATCGATGCGGCGCTCGTCGCCGAAGGGCTCGTCGAGAAGGTCGTGCGTGCGGCGGCCGAGCTGTCGCGGCGGCT
GAACTATCGGTCCGACGACGAAAACACCTATTTGAAGGCATTGGGATTGCGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A3NIY6

Antitoxin

Source ID Structure
AlphaFold DB A0A3N4FRW8

References