Detailed information of TA system TA120816

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 3012529..3013151
• Replicon: chromosome
• Accession: NZ_CP038213
• Organism: Ornithinimicrobium flavum strain CPCC 203535

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: E3Z34_RS14165
• Protein ID: WP_202976961.1
• Coordinates: 3012529..3012939 (-)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: E3Z34_RS14170
• Protein ID: WP_134774123.1
• Coordinates: 3012930..3013151 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z34_RS14140	3007819..3008934	+	1116	WP_022921923.1	tyrosine-type recombinase/integrase	-
E3Z34_RS14145	3008931..3009266	+	336	WP_022921924.1	helix-turn-helix transcriptional regulator	-
E3Z34_RS14150	3009419..3010726	+	1308	WP_134774922.1	hypothetical protein	-
E3Z34_RS14155	3010794..3011144	+	351	WP_029202581.1	metalloregulator ArsR/SmtB family transcription factor	-
E3Z34_RS14160	3011141..3011737	+	597	WP_022921927.1	cadmium resistance transporter	-
E3Z34_RS14165	3012529..3012939	-	411	WP_202976961.1	PIN domain nuclease	Toxin
E3Z34_RS14170	3012930..3013151	-	222	WP_134774123.1	antitoxin	Antitoxin
E3Z34_RS14175	3013255..3014178	-	924	WP_158288690.1	tyrosine-type recombinase/integrase	-
E3Z34_RS14180	3014156..3014965	+	810	WP_202976962.1	hypothetical protein	-
E3Z34_RS14185	3015081..3015665	+	585	WP_134774126.1	hypothetical protein	-
E3Z34_RS14190	3015669..3016007	+	339	WP_134774127.1	DUF3024 domain-containing protein	-
E3Z34_RS14195	3016227..3016991	+	765	Protein_2809	ISL3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	2965989..3037844	71855

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-124)

Antitoxin

• Ribbon-helix-helix protein, CopG (9-34)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14910.15 Da
• Isoelectric Point: 4.9622

>T120816 WP_202976961.1 NZ_CP038213:c3012939-3012529 [Ornithinimicrobium flavum]
VAVTDWLIDKSALVRLGASPDAAEWAGRIERGLVRITTMTRLEVGYSARTAADARSVFGTPPISMMPVEYLTPAIEDRAL
EVQLLLADRGRHRAPSIPDLLIAATAELAGLTVLHLDKDFELIEELTGQHTERLRP

Nucleotide

• Length: 411 bp

>T120816 NZ_CP038213:c3012939-3012529 [Ornithinimicrobium flavum]
GTGGCGGTGACTGACTGGCTGATTGACAAGTCCGCCTTGGTCCGATTGGGCGCCAGTCCCGACGCCGCCGAGTGGGCTGG
GCGGATCGAACGAGGGCTCGTCCGGATCACCACCATGACCAGACTAGAGGTTGGTTACTCGGCGCGGACCGCCGCGGACG
CGCGGTCGGTGTTCGGCACCCCGCCGATCTCGATGATGCCGGTCGAGTACCTGACACCCGCGATCGAGGACCGAGCCCTC
GAGGTCCAGCTGCTGCTGGCTGACAGGGGCCGTCACCGGGCCCCGTCAATCCCGGACCTGCTCATCGCCGCTACCGCTGA
GCTCGCGGGTCTGACGGTGCTGCACCTCGACAAGGACTTCGAGCTCATCGAGGAACTGACCGGTCAGCACACCGAGCGCC
TCCGCCCTTGA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7979.00 Da
• Isoelectric Point: 5.0041

>AT120816 WP_134774123.1 NZ_CP038213:c3013151-3012930 [Ornithinimicrobium flavum]
MADVLIRDVPEAVLAGVDAHAARLGLSRAEYIRRRLASDAATASAPVSVEVLRAFADRFADLTDHQTMDEAWR

Nucleotide

• Length: 222 bp

>AT120816 NZ_CP038213:c3013151-3012930 [Ornithinimicrobium flavum]
ATGGCTGACGTGCTGATTCGCGATGTTCCTGAGGCGGTGCTGGCCGGCGTGGATGCCCATGCGGCCCGACTCGGGCTCTC
CAGGGCCGAGTACATCCGCAGACGCTTGGCCTCGGACGCCGCCACCGCGAGCGCGCCCGTCTCCGTCGAGGTTCTCCGTG
CCTTCGCGGACAGGTTCGCCGACCTCACCGACCACCAGACGATGGACGAAGCGTGGCGGTGA