Detailed information of TA system TA120813

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 356919..357475
• Replicon: chromosome
• Accession: NZ_CP038213
• Organism: Ornithinimicrobium flavum strain CPCC 203535

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3Z34_RS01680
• Protein ID: WP_022920828.1
• Coordinates: 356919..357239 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: -
• Locus tag: E3Z34_RS01685
• Protein ID: WP_134772214.1
• Coordinates: 357242..357475 (-)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z34_RS01640	352791..353435	-	645	WP_158288576.1	hypothetical protein	-
E3Z34_RS01645	353538..354242	-	705	WP_158288577.1	class I SAM-dependent methyltransferase	-
E3Z34_RS01650	354367..354609	-	243	WP_134772210.1	hypothetical protein	-
E3Z34_RS01655	354628..354855	-	228	WP_202976999.1	hypothetical protein	-
E3Z34_RS01660	354933..355862	+	930	WP_134772211.1	tyrosine-type recombinase/integrase	-
E3Z34_RS01665	355947..356195	+	249	WP_122260808.1	ribbon-helix-helix protein, CopG family	-
E3Z34_RS01670	356192..356434	+	243	WP_134772212.1	toxin	-
E3Z34_RS01675	356522..356821	-	300	WP_134772213.1	hypothetical protein	-
E3Z34_RS01680	356919..357239	-	321	WP_022920828.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3Z34_RS01685	357242..357475	-	234	WP_134772214.1	hypothetical protein	Antitoxin
E3Z34_RS01690	359144..359635	+	492	WP_134772215.1	transposase	-
E3Z34_RS01695	360159..361466	-	1308	WP_134772184.1	ISL3 family transposase	-
E3Z34_RS01700	361626..362198	+	573	WP_134772216.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	351351..371417	20066
	-	flank	IS/Tn	-	-	360159..361466	1307

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (10-93)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 11512.40 Da
• Isoelectric Point: 5.6982

>T120813 WP_022920828.1 NZ_CP038213:c357239-356919 [Ornithinimicrobium flavum]
MREICLARLDKTRPVLVLTRDTARAVMTKVTVAPITSTVKGLSSEVPVGRANGLDHDSAVSIDNVLTIPADLLGRTLGFL
TPEQERHLARAIVLAYDLEVPLLEEP

Nucleotide

• Length: 321 bp

>T120813 NZ_CP038213:c357239-356919 [Ornithinimicrobium flavum]
TTGCGCGAGATCTGCCTGGCGAGGCTGGACAAGACGCGCCCCGTCCTGGTGCTCACCCGTGACACTGCCCGCGCCGTGAT
GACCAAGGTGACCGTGGCTCCCATCACGTCGACTGTCAAGGGGTTGTCCAGCGAAGTACCGGTAGGCCGTGCTAACGGGC
TGGACCACGACAGTGCTGTCTCGATCGACAACGTCCTGACCATCCCCGCCGACCTCCTGGGCAGGACGCTGGGGTTCCTC
ACGCCCGAGCAGGAGCGCCACCTGGCAAGAGCCATCGTGCTGGCGTACGACCTCGAGGTGCCGCTGCTCGAAGAGCCGTG
A

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8320.45 Da
• Isoelectric Point: 5.0176

>AT120813 WP_134772214.1 NZ_CP038213:c357475-357242 [Ornithinimicrobium flavum]
VTTQIAVRLPDEMVRFLDRSVASGKAASRAALVAAALEREMRVQAAQQDVQTLKKKGTADDLDELVAWSVAHATLDD

Nucleotide

• Length: 234 bp

>AT120813 NZ_CP038213:c357475-357242 [Ornithinimicrobium flavum]
GTGACGACACAGATCGCGGTACGGCTCCCTGACGAGATGGTTCGCTTTCTGGACCGGAGCGTCGCCAGTGGCAAGGCTGC
CAGCCGAGCCGCGCTGGTGGCCGCGGCCCTGGAGCGCGAGATGCGCGTGCAGGCCGCGCAGCAGGACGTCCAGACCCTGA
AGAAGAAGGGCACGGCCGATGACCTCGACGAGCTCGTCGCCTGGTCCGTGGCCCACGCGACACTGGACGACTGA