Detailed information of TA system TA120807

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2373808..2374424
• Replicon: chromosome
• Accession: NZ_CP038207
• Organism: Pseudomonas sp. S150

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: E3Z29_RS10975
• Protein ID: WP_134785765.1
• Coordinates: 2373808..2374020 (+)
• Length: 71 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: W6W5D4
• Locus tag: E3Z29_RS10980
• Protein ID: WP_007961664.1
• Coordinates: 2374020..2374424 (+)
• Length: 135 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3Z29_RS10950	2369911..2370447	+	537	WP_041072930.1	DsbE family thiol:disulfide interchange protein	-
E3Z29_RS10955	2370444..2370914	+	471	WP_003222948.1	cytochrome c-type biogenesis protein CcmH	-
E3Z29_RS10960	2370911..2372113	+	1203	WP_134785763.1	c-type cytochrome biogenesis protein CcmI	-
E3Z29_RS10965	2372141..2372545	+	405	WP_134785764.1	hypothetical protein	-
E3Z29_RS10970	2372652..2373540	-	889	Protein_2109	LysR family transcriptional regulator	-
E3Z29_RS10975	2373808..2374020	+	213	WP_134785765.1	type II toxin-antitoxin system HicA family toxin	Toxin
E3Z29_RS10980	2374020..2374424	+	405	WP_007961664.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
E3Z29_RS10985	2374483..2375670	-	1188	WP_096796242.1	MFS transporter	-
E3Z29_RS10990	2375914..2376534	+	621	WP_134785766.1	hypothetical protein	-
E3Z29_RS10995	2376814..2378091	+	1278	WP_134785767.1	hypothetical protein	-
E3Z29_RS11000	2378078..2379025	+	948	WP_127646222.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (6-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-127)

Sequences

Toxin

Protein

• Length: 71 a.a.
• Molecular weight: 8025.26 Da
• Isoelectric Point: 10.3701

>T120807 WP_134785765.1 NZ_CP038207:2373808-2374020 [Pseudomonas sp. S150]
VQSRLLIKELEEAGWTLDRVIGSHHIFTHRYNPYTIPVPHPKKDLPLGTVKSIRRRAGLYHPPASYKGDP

Nucleotide

• Length: 213 bp

>T120807 NZ_CP038207:2373808-2374020 [Pseudomonas sp. S150]
GTGCAAAGCAGGCTATTGATCAAGGAGCTGGAGGAGGCAGGCTGGACGCTGGATCGAGTCATCGGCAGTCATCACATCTT
CACGCATCGTTACAACCCGTACACCATTCCCGTTCCTCACCCTAAAAAGGATTTGCCGCTGGGGACGGTCAAAAGCATCA
GGAGGCGTGCCGGGTTGTACCACCCGCCAGCCAGCTACAAGGGAGATCCATAA

Antitoxin

Protein

• Length: 135 a.a.
• Molecular weight: 14836.80 Da
• Isoelectric Point: 4.2870

>AT120807 WP_007961664.1 NZ_CP038207:2374020-2374424 [Pseudomonas sp. S150]
MQYPICIEWGDDNTAIGIQIPDIPGAVTAGDTFEDAYNAAVEVAHLMLQEMAADGESIPMPTSAAAHRNNPEFADMGWGM
LELDISPYMGKTEKVNVTLPGYVIQRIDRYVREHNVKSRSSFLADAAMEKLVRY

Nucleotide

• Length: 405 bp

>AT120807 NZ_CP038207:2374020-2374424 [Pseudomonas sp. S150]
ATGCAATATCCGATCTGCATCGAATGGGGTGACGACAACACCGCCATCGGTATTCAGATACCTGACATTCCCGGCGCCGT
CACGGCCGGGGACACTTTCGAGGACGCCTATAACGCAGCCGTTGAAGTCGCCCATCTCATGTTGCAGGAGATGGCGGCGG
ACGGGGAATCGATTCCGATGCCGACGTCGGCAGCGGCCCATCGCAATAATCCGGAGTTTGCCGACATGGGCTGGGGGATG
CTCGAGCTGGATATCTCGCCGTACATGGGCAAGACCGAGAAGGTCAACGTGACGTTGCCAGGCTATGTGATCCAGCGCAT
CGATCGCTATGTGCGTGAACACAATGTCAAAAGCCGCTCTTCGTTTCTGGCAGATGCGGCGATGGAGAAGCTGGTTCGGT
ATTAA

Structures

Antitoxin

• AlphaFold DB: W6W5D4