Detailed information of TA system TA120805

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 433679..434214
• Replicon: plasmid pPAN2
• Accession: NZ_CP038205
• Organism: Paracoccus pantotrophus strain DSM 11072

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: A0A1I5P2D9
• Locus tag: E3U24_RS13125
• Protein ID: WP_024846182.1
• Coordinates: 433915..434214 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: A0A1I5P292
• Locus tag: E3U24_RS13120
• Protein ID: WP_028711008.1
• Coordinates: 433679..433915 (+)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3U24_RS13100 (E3U24_12960)	428954..429307	+	354	WP_114669553.1	IS66 family insertion sequence element accessory protein TnpB	-
E3U24_RS13105 (E3U24_12965)	429362..430918	+	1557	WP_281029332.1	IS66 family transposase	-
E3U24_RS13110 (E3U24_12970)	431172..432740	+	1569	WP_281029367.1	IS21 family transposase	-
E3U24_RS13115 (E3U24_12975)	432670..433398	+	729	WP_024843951.1	IS21-like element ISPkr1 family helper ATPase IstB	-
E3U24_RS13120 (E3U24_12980)	433679..433915	+	237	WP_028711008.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
E3U24_RS13125 (E3U24_12985)	433915..434214	+	300	WP_024846182.1	CcdB family protein	Toxin
E3U24_RS13130 (E3U24_12990)	434678..434914	-	237	WP_024846181.1	hypothetical protein	-
E3U24_RS13135 (E3U24_12995)	434915..435136	+	222	Protein_417	transposase domain-containing protein	-
E3U24_RS13140 (E3U24_13000)	435118..435312	+	195	WP_024846179.1	TonB-dependent receptor	-
E3U24_RS13145 (E3U24_13005)	435569..436183	+	615	WP_231487003.1	hypothetical protein	-
E3U24_RS13150 (E3U24_13010)	436180..437205	+	1026	WP_179922270.1	iron chelate uptake ABC transporter family permease subunit	-
E3U24_RS13155 (E3U24_13015)	437862..438200	-	339	WP_281029368.1	hypothetical protein	-
E3U24_RS13160 (E3U24_13020)	438322..438990	-	669	WP_024844137.1	ABC transporter ATP-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	glf	1..538229	538229

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (3-98)

Antitoxin

• Post-segregation antitoxin CcdA (8-78)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 10669.40 Da
• Isoelectric Point: 4.1564

>T120805 WP_024846182.1 NZ_CP038205:433915-434214 [Paracoccus pantotrophus]
MARYDVYASPDGSGYLLDVQTDLLDNLSTRLVVPMMPVGASPPVLKQLNPIFDIQSERYVMVTQFMAAVPASILKTLAGS
LAQHDTEITGALDMLLTGV

Nucleotide

• Length: 300 bp

>T120805 NZ_CP038205:433915-434214 [Paracoccus pantotrophus]
ATGGCGCGATATGACGTTTATGCCAGCCCGGATGGTTCGGGGTATCTTCTCGATGTGCAGACCGACCTTCTGGACAATCT
GAGCACGCGCCTTGTGGTGCCGATGATGCCGGTGGGCGCTTCGCCGCCCGTGCTCAAGCAGCTGAACCCCATCTTTGACA
TACAGTCCGAGCGCTATGTGATGGTGACGCAGTTCATGGCCGCGGTTCCCGCTTCCATTCTGAAGACACTCGCAGGCAGC
CTTGCACAGCACGATACCGAGATCACGGGCGCGCTGGACATGCTCCTGACCGGGGTGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8753.95 Da
• Isoelectric Point: 10.2690

>AT120805 WP_028711008.1 NZ_CP038205:433679-433915 [Paracoccus pantotrophus]
MQTQSVPKKPTNLSLDQGLLSEARSFGVNLSQAAEAGLRRAVREAKAEAWKRENAKAIEGVNRWVEENGLPLEKYRLF

Nucleotide

• Length: 237 bp

>AT120805 NZ_CP038205:433679-433915 [Paracoccus pantotrophus]
ATGCAGACACAATCCGTTCCCAAAAAACCGACCAATCTGTCACTGGATCAGGGCTTGCTCAGCGAAGCCCGGTCCTTCGG
AGTAAACCTGTCGCAGGCCGCAGAGGCCGGTTTGCGTCGCGCGGTCAGGGAAGCCAAAGCCGAGGCGTGGAAGCGTGAGA
ACGCCAAGGCGATTGAAGGGGTTAACCGTTGGGTCGAAGAGAACGGCCTGCCCCTTGAAAAATACCGGCTCTTCTGA

Structures

Toxin

• AlphaFold DB: A0A1I5P2D9

Antitoxin

• AlphaFold DB: A0A1I5P292