Detailed information of TA system TA120793

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT_toxin-BrnA
• Location: 511960..512401
• Replicon: chromosome
• Accession: NZ_CP038194
• Organism: Burkholderia pseudomallei strain BPs122

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: -
• Locus tag: E3O37_RS02720
• Protein ID: WP_009939402.1
• Coordinates: 512228..512401 (-)
• Length: 58 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: Q63NA3
• Locus tag: E3O37_RS02715
• Protein ID: WP_004525420.1
• Coordinates: 511960..512244 (-)
• Length: 95 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3O37_RS02675	507165..509180	+	2016	WP_004525415.1	cation acetate symporter	-
E3O37_RS36505	509300..509917	-	618	WP_167751092.1	toprim domain-containing protein	-
E3O37_RS36510	509815..510375	-	561	WP_167751103.1	PriCT-2 domain-containing protein	-
E3O37_RS02690	510479..510580	-	102	WP_134778823.1	splicing coactivator subunit	-
E3O37_RS02695	510596..510900	+	305	Protein_423	recombinase family protein	-
E3O37_RS02700	510907..511314	-	408	Protein_424	DDE-type integrase/transposase/recombinase	-
E3O37_RS02715	511960..512244	-	285	WP_004525420.1	BrnA antitoxin family protein	Antitoxin
E3O37_RS02720	512228..512401	-	174	WP_009939402.1	BrnT family toxin	Toxin
E3O37_RS02725	513069..513344	+	276	WP_004552388.1	bacteriophage protein Gp49	-
E3O37_RS02730	513354..513485	+	132	WP_009923452.1	bacteriophage protein Gp48	-
E3O37_RS36515	513673..513891	+	219	WP_004552392.1	hypothetical protein	-
E3O37_RS36520	514093..514410	+	318	WP_166682741.1	hypothetical protein	-
E3O37_RS02750	514407..515180	+	774	Protein_431	prohibitin family protein	-
E3O37_RS37360	515178..515582	+	405	Protein_432	DDE-type integrase/transposase/recombinase	-
E3O37_RS02755	515545..515973	+	429	Protein_433	IS110 family transposase	-
E3O37_RS02760	516200..516316	-	117	Protein_434	IS3 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	507165..521633	14468
	-	inside	IScluster/Tn	-	-	510907..521633	10726

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (4-49)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (27-88)

Sequences

Toxin

Protein

• Length: 58 a.a.
• Molecular weight: 6691.59 Da
• Isoelectric Point: 7.9950

>T120793 WP_009939402.1 NZ_CP038194:c512401-512228 [Burkholderia pseudomallei]
MSYVDDRRDYSEVREVGFGVIGDRLYCVVFTQRGDSMHIISMRKANKREVKSYVEQA

Nucleotide

• Length: 174 bp

>T120793 NZ_CP038194:c512401-512228 [Burkholderia pseudomallei]
TTGTCCTACGTGGACGACCGACGCGACTACAGTGAAGTGCGCGAAGTCGGGTTCGGTGTGATCGGCGATCGCCTCTATTG
CGTGGTGTTCACGCAGCGCGGCGACTCGATGCACATCATCAGCATGCGTAAGGCGAACAAGCGGGAGGTCAAGAGCTATG
TCGAGCAAGCGTAA

Antitoxin

Protein

• Length: 95 a.a.
• Molecular weight: 10697.10 Da
• Isoelectric Point: 5.8930

>AT120793 WP_004525420.1 NZ_CP038194:c512244-511960 [Burkholderia pseudomallei]
MSSKRKIVMPTDEEDAAINRGIAADPDTFEVPAEDFAKMTRRGKRGRPPLEAPKVQLTVRYDVDIVDAFKATGEGWQTRM
NDALREWLREHQPA

Nucleotide

• Length: 285 bp

>AT120793 NZ_CP038194:c512244-511960 [Burkholderia pseudomallei]
ATGTCGAGCAAGCGTAAGATCGTCATGCCGACGGACGAGGAAGACGCGGCAATCAACCGCGGCATCGCGGCCGATCCCGA
CACGTTCGAAGTGCCGGCGGAAGACTTCGCGAAGATGACGCGGCGAGGCAAGCGTGGCCGTCCGCCGCTCGAAGCGCCCA
AGGTGCAATTGACGGTGCGCTATGACGTCGACATTGTCGACGCCTTCAAAGCGACGGGCGAAGGTTGGCAGACGCGTATG
AATGATGCGTTGCGCGAGTGGCTGAGGGAACATCAGCCTGCGTAG

Structures

Antitoxin

• AlphaFold DB: A0A0F6L9S8