Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 764023..764659 Replicon chromosome
Accession NZ_CP038186
Organism Bacillus licheniformis strain MCC 2514

Toxin (Protein)


Gene name mazF Uniprot ID M5P3Q9
Locus tag EYQ98_RS03745 Protein ID WP_003179128.1
Coordinates 764309..764659 (+) Length 117 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID M5PDU2
Locus tag EYQ98_RS03740 Protein ID WP_006638778.1
Coordinates 764023..764304 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
EYQ98_RS03720 759138..760619 + 1482 WP_009330132.1 PH domain-containing protein -
EYQ98_RS03725 760616..761215 - 600 WP_003179118.1 rhomboid family intramembrane serine protease -
EYQ98_RS03730 761558..762517 + 960 WP_152847888.1 outer membrane lipoprotein carrier protein LolA -
EYQ98_RS03735 762742..763911 + 1170 WP_026080763.1 alanine racemase -
EYQ98_RS03740 764023..764304 + 282 WP_006638778.1 hypothetical protein Antitoxin
EYQ98_RS03745 764309..764659 + 351 WP_003179128.1 type II toxin-antitoxin system endoribonuclease NdoA Toxin
EYQ98_RS03750 764777..765604 + 828 WP_003179130.1 RsbT co-antagonist protein RsbRA -
EYQ98_RS03755 765608..765973 + 366 WP_003179132.1 STAS domain-containing protein -
EYQ98_RS03760 765976..766377 + 402 WP_003179135.1 anti-sigma regulatory factor -
EYQ98_RS03765 766388..767395 + 1008 WP_003179137.1 PP2C family protein-serine/threonine phosphatase -
EYQ98_RS03770 767454..767780 + 327 WP_003179140.1 anti-sigma factor antagonist -
EYQ98_RS03775 767780..768265 + 486 WP_003179142.1 anti-sigma B factor RsbW -
EYQ98_RS03780 768231..769022 + 792 WP_003179144.1 RNA polymerase sigma factor SigB -
EYQ98_RS03785 769019..769618 + 600 WP_003179145.1 SpoIIE family protein phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 117 a.a.        Molecular weight: 12990.03 Da        Isoelectric Point: 5.7234

>T120791 WP_003179128.1 NZ_CP038186:764309-764659 [Bacillus licheniformis]
MIVKRGDVYFADLSPVVGSEQGGVRPVLVIQNNIGNRFSPTAIVAAITAQIQKAKLPTHVEIDAKRYGFERDSVILLEQI
RTIDKQRLTDKITHLDDEMMEKVNEALQISLALIDF

Download         Length: 351 bp

>T120791 NZ_CP038186:764309-764659 [Bacillus licheniformis]
TTGATTGTTAAACGCGGCGATGTTTATTTTGCTGACCTATCTCCTGTTGTTGGCTCAGAACAAGGCGGGGTGCGCCCGGT
TTTAGTGATTCAAAACAACATCGGCAATCGCTTCAGCCCAACTGCTATTGTTGCAGCCATAACAGCCCAAATACAGAAAG
CAAAATTACCTACCCACGTCGAAATTGATGCGAAACGCTACGGTTTTGAAAGAGACTCCGTTATATTGCTCGAACAAATT
CGGACGATTGACAAGCAAAGATTAACGGACAAAATCACCCATCTCGATGATGAAATGATGGAAAAGGTCAACGAAGCCTT
ACAAATCAGTTTGGCACTCATTGATTTTTAA

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10649.03 Da        Isoelectric Point: 4.6920

>AT120791 WP_006638778.1 NZ_CP038186:764023-764304 [Bacillus licheniformis]
MSESSATTEILIRLPEALVSELDGVVMQDNGSRNELIYQATKMYLRERKNRQIRESMRRGYMEMAKINLNISSEAQFAEY
EAENTVERLVSGG

Download         Length: 282 bp

>AT120791 NZ_CP038186:764023-764304 [Bacillus licheniformis]
TTGTCTGAATCCAGCGCAACAACTGAAATATTGATTCGCTTGCCAGAAGCTTTAGTATCAGAACTGGATGGTGTCGTCAT
GCAAGATAACGGGAGCAGAAATGAACTGATTTACCAAGCCACAAAAATGTATTTACGCGAACGCAAAAATCGACAAATTC
GGGAATCGATGAGACGCGGTTATATGGAGATGGCCAAGATCAATTTGAACATCTCTTCTGAGGCTCAATTTGCAGAGTAT
GAGGCTGAAAACACAGTAGAGCGCTTAGTAAGCGGAGGATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A6I7FHI4

Antitoxin

Source ID Structure
AlphaFold DB M5PDU2

References