Detailed information of TA system TA120783

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2109661..2110190
• Replicon: chromosome
• Accession: NZ_CP038183
• Organism: Staphylococcus aureus strain MJ015

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3306_RS10440
• Protein ID: WP_000621175.1
• Coordinates: 2109661..2110023 (-)
• Length: 121 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: T1YCG8
• Locus tag: E3306_RS10445
• Protein ID: WP_000948331.1
• Coordinates: 2110020..2110190 (-)
• Length: 57 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3306_RS10420	2106640..2107410	-	771	WP_001041102.1	RNA polymerase sigma factor SigB	-
E3306_RS10425	2107385..2107864	-	480	WP_001190829.1	anti-sigma B factor RsbW	-
E3306_RS10430	2107866..2108192	-	327	WP_001052491.1	anti-sigma factor antagonist	-
E3306_RS10435	2108311..2109312	-	1002	WP_061839378.1	PP2C family protein-serine/threonine phosphatase	-
E3306_RS10440	2109661..2110023	-	363	WP_000621175.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3306_RS10445	2110020..2110190	-	171	WP_000948331.1	type II toxin-antitoxin system antitoxin MazE	Antitoxin
E3306_RS10450	2110275..2111423	-	1149	WP_001281151.1	alanine racemase	-
E3306_RS10455	2111489..2111848	-	360	WP_000581200.1	holo-ACP synthase	-
E3306_RS10460	2111852..2112343	-	492	WP_001205910.1	PH domain-containing protein	-
E3306_RS10465	2112330..2113913	-	1584	WP_001294626.1	PH domain-containing protein	-
E3306_RS10470	2113906..2114385	-	480	WP_001287090.1	hypothetical protein	-
E3306_RS10475	2114594..2115154	-	561	WP_001092416.1	K(+)-transporting ATPase subunit C	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 121 a.a.
• Molecular weight: 13441.69 Da
• Isoelectric Point: 10.1654

>T120783 WP_000621175.1 NZ_CP038183:c2110023-2109661 [Staphylococcus aureus]
MIRRGDVYLADLSPVQGSEQGGVRPVVIIQNDTGNKYSPTVIVAAITGRINKAKIPTHVEIEKKKYKLDKDSVILLEQIR
TLDKKRLKEKLTYLSDDKMKEVDNALMISLGLNAVAHQKN

Nucleotide

• Length: 363 bp

>T120783 NZ_CP038183:c2110023-2109661 [Staphylococcus aureus]
ATGATTAGACGAGGAGATGTTTATTTAGCAGATTTATCACCAGTACAGGGATCTGAACAAGGGGGAGTCAGACCTGTAGT
CATAATTCAAAATGATACTGGTAATAAATATAGTCCTACAGTTATTGTTGCGGCAATAACTGGTAGGATTAATAAAGCGA
AAATACCGACACATGTAGAGATTGAAAAGAAAAAGTATAAGTTGGATAAAGACTCAGTTATATTATTAGAACAAATTCGT
ACACTTGATAAAAAACGATTGAAAGAAAAACTGACGTACTTATCCGATGATAAAATGAAAGAAGTAGATAATGCACTAAT
GATTAGTTTAGGGCTGAATGCAGTAGCTCACCAGAAAAATTAG

Antitoxin

Protein

• Length: 57 a.a.
• Molecular weight: 6251.78 Da
• Isoelectric Point: 3.8305

>AT120783 WP_000948331.1 NZ_CP038183:c2110190-2110020 [Staphylococcus aureus]
MLSFSQNRSHSLEQSLKEGYSQMADLNLSLANEAFPIECEACDCNETYLSSNSTNE

Nucleotide

• Length: 171 bp

>AT120783 NZ_CP038183:c2110190-2110020 [Staphylococcus aureus]
ATGTTATCTTTTAGTCAAAATAGAAGTCATAGCTTAGAACAATCTTTAAAAGAAGGATATTCACAAATGGCTGATTTAAA
TCTCTCCCTAGCGAACGAAGCTTTTCCGATAGAGTGTGAAGCATGCGATTGCAACGAAACATATTTATCTTCTAATTCAA
CGAATGAATGA

Structures

Antitoxin

• AlphaFold DB: A0A0E0VRZ1