Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/-
Location 28704..29296 Replicon 2 HS-C plasmid p2HS-C-1
Accession NZ_CP038181
Organism Escherichia coli strain

Toxin (Protein)


Gene name hicA Uniprot ID V0AGQ2
Locus tag E4F32_RS22260 Protein ID WP_000520549.1
Coordinates 28704..28952 (+) Length 83 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID V0AWG0
Locus tag E4F32_RS22265 Protein ID WP_000681613.1
Coordinates 28949..29296 (+) Length 116 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4F32_RS22225 25581..26096 + 516 WP_001025395.1 J domain-containing protein -
E4F32_RS22230 26201..26743 + 543 WP_001083906.1 hypothetical protein -
E4F32_RS22235 26740..26991 + 252 WP_001230707.1 hypothetical protein -
E4F32_RS22240 26978..27205 + 228 WP_000801440.1 hypothetical protein -
E4F32_RS22250 27659..27958 + 300 WP_000650309.1 hypothetical protein -
E4F32_RS22255 28049..28399 + 351 WP_000854259.1 hypothetical protein -
E4F32_RS22260 28704..28952 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin Toxin
E4F32_RS22265 28949..29296 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin Antitoxin
E4F32_RS22275 29657..30160 + 504 WP_000781813.1 hypothetical protein -
E4F32_RS22280 30164..31393 + 1230 WP_134778717.1 relaxase/mobilization nuclease domain-containing protein -
E4F32_RS22285 31470..31763 + 294 WP_060598542.1 LuxR family transcriptional regulator -
E4F32_RS22290 31760..31975 + 216 WP_000760375.1 EexN family lipoprotein -
E4F32_RS22295 32331..32951 + 621 WP_000157549.1 lytic transglycosylase domain-containing protein -
E4F32_RS22300 33019..33291 + 273 WP_001328551.1 TrbC/VirB2 family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33309 33309


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(20-81)

Antitoxin

Uncharacterised protein family HicB

(54-104)


Sequences

Toxin        


Download         Length: 83 a.a.        Molecular weight: 9422.08 Da        Isoelectric Point: 10.4800

>T120772 WP_000520549.1 NZ_CP038181:28704-28952 [Escherichia coli]
MGKTDKLLAKFLNSKKTFEWDELVVLFSSLGYVKKEMQGSRVRFFNAEINHTILMHRPHPESYIKGGTLKAIKQNLKEAG
LL

Download         Length: 249 bp

>T120772 NZ_CP038181:28704-28952 [Escherichia coli]
ATGGGGAAAACAGATAAGCTACTGGCAAAGTTTTTAAACAGTAAAAAAACGTTTGAATGGGATGAACTGGTCGTTTTGTT
TTCCTCTTTGGGATATGTCAAAAAGGAAATGCAGGGGTCAAGAGTGCGGTTTTTCAATGCTGAAATCAATCACACCATAT
TAATGCATCGTCCACATCCGGAAAGTTATATCAAAGGTGGTACGCTGAAAGCAATTAAACAGAATCTGAAAGAGGCTGGA
TTATTATGA

Antitoxin


Download         Length: 116 a.a.        Molecular weight: 13113.84 Da        Isoelectric Point: 4.6723

>AT120772 WP_000681613.1 NZ_CP038181:28949-29296 [Escherichia coli]
MKHLKYKGYLGTVEPDFENNVLYGKLAFIRDLVTYEASTLAELEQEFKTSVDLYLQSCVEDGKEPDTPFKGVFNVRLDPE
LHRRVAEMAMEEDLSLNAFVNKALEKEVSNHRAGA

Download         Length: 348 bp

>AT120772 NZ_CP038181:28949-29296 [Escherichia coli]
ATGAAACATTTAAAATATAAAGGATATTTAGGCACGGTTGAACCCGATTTTGAAAATAATGTCCTGTATGGAAAACTGGC
GTTTATTCGGGACCTGGTAACTTATGAGGCTTCTACATTAGCTGAACTGGAACAGGAGTTTAAAACATCTGTTGATCTGT
ATTTACAGTCTTGTGTGGAGGATGGAAAGGAACCTGATACCCCGTTTAAAGGCGTGTTTAACGTCAGGCTTGATCCAGAA
CTGCATCGTCGGGTAGCAGAAATGGCGATGGAAGAAGATTTATCGTTGAATGCCTTTGTTAATAAGGCTCTGGAAAAAGA
AGTCAGCAATCATCGCGCAGGGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829CIX3

Antitoxin

Source ID Structure
AlphaFold DB A0A829CLC1

References