120771

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) TsxAB/MqsA(antitoxin)
Location 26740..27205 Replicon 2 HS-C plasmid p2HS-C-1
Accession NZ_CP038181
Organism Escherichia coli strain

Toxin (Protein)


Gene name TsxA Uniprot ID U9ZUY2
Locus tag E4F32_RS22235 Protein ID WP_001230707.1
Coordinates 26740..26991 (+) Length 84 a.a.

Antitoxin (Protein)


Gene name TsxB Uniprot ID M7CCF8
Locus tag E4F32_RS22240 Protein ID WP_000801440.1
Coordinates 26978..27205 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4F32_RS22210 22221..22748 - 528 WP_000577043.1 DUF2726 domain-containing protein -
E4F32_RS22215 22883..23287 - 405 WP_012421203.1 hypothetical protein -
E4F32_RS22220 23300..24166 - 867 WP_001353743.1 replication initiation protein -
E4F32_RS22225 25581..26096 + 516 WP_001025395.1 J domain-containing protein -
E4F32_RS22230 26201..26743 + 543 WP_001083906.1 hypothetical protein -
E4F32_RS22235 26740..26991 + 252 WP_001230707.1 hypothetical protein Toxin
E4F32_RS22240 26978..27205 + 228 WP_000801440.1 hypothetical protein Antitoxin
E4F32_RS22250 27659..27958 + 300 WP_000650309.1 hypothetical protein -
E4F32_RS22255 28049..28399 + 351 WP_000854259.1 hypothetical protein -
E4F32_RS22260 28704..28952 + 249 WP_000520549.1 type II toxin-antitoxin system HicA family toxin -
E4F32_RS22265 28949..29296 + 348 WP_000681613.1 type II toxin-antitoxin system HicB family antitoxin -
E4F32_RS22275 29657..30160 + 504 WP_000781813.1 hypothetical protein -
E4F32_RS22280 30164..31393 + 1230 WP_134778717.1 relaxase/mobilization nuclease domain-containing protein -
E4F32_RS22285 31470..31763 + 294 WP_060598542.1 LuxR family transcriptional regulator -
E4F32_RS22290 31760..31975 + 216 WP_000760375.1 EexN family lipoprotein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid mcr-1.1 - 1..33309 33309


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(27-151)

Antitoxin

Vibrio phage ICP1, Orf50

(6-82)


Sequences

Toxin        


Download         Length: 84 a.a.        Molecular weight: 9751.13 Da        Isoelectric Point: 8.9187

>T120771 WP_001230707.1 NZ_CP038181:26740-26991 [Escherichia coli]
MRKNKGDVTYFLEKEGDNYRLTKRIKARTNVKIGNKTTKITLYDAVLNENELQHIDFTCAGLREDDETPVKNLIKEFMLN
ETR

Download         Length: 252 bp

>T120771 NZ_CP038181:26740-26991 [Escherichia coli]
ATGAGAAAAAATAAAGGTGATGTGACTTATTTTCTTGAAAAAGAAGGTGACAATTACAGGCTTACAAAAAGGATAAAAGC
CAGAACAAATGTTAAAATCGGGAACAAAACTACGAAAATTACATTGTATGATGCTGTACTGAATGAGAATGAATTACAAC
ATATTGATTTTACGTGTGCCGGATTAAGGGAAGACGATGAGACGCCAGTTAAGAATTTAATTAAGGAGTTTATGTTAAAT
GAAACCCGATAA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8659.98 Da        Isoelectric Point: 8.5105

>AT120771 WP_000801440.1 NZ_CP038181:26978-27205 [Escherichia coli]
MKPDNTPENVKKLRLKAGLTQKECSHIYGVGLRTWQKKEEVNTQNSQSLSLVEFEFLLLLAGEHPEYVLCKRESK

Download         Length: 228 bp

>AT120771 NZ_CP038181:26978-27205 [Escherichia coli]
ATGAAACCCGATAATACACCTGAAAATGTAAAAAAATTACGTTTAAAAGCAGGATTAACACAAAAGGAATGCAGTCATAT
TTATGGTGTTGGGCTAAGAACCTGGCAGAAAAAAGAGGAAGTTAATACTCAAAACAGTCAAAGTCTGTCACTGGTTGAGT
TTGAGTTTCTTTTGTTACTTGCAGGAGAACATCCTGAATACGTGCTCTGCAAAAGGGAGTCAAAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB U9ZUY2

Antitoxin

Source ID Structure
AlphaFold DB A0A5Y6U8W9

References