Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3349026..3349644 Replicon chromosome
Accession NZ_CP038180
Organism Escherichia coli strain

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag E4F32_RS16340 Protein ID WP_001291435.1
Coordinates 3349426..3349644 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag E4F32_RS16335 Protein ID WP_000344800.1
Coordinates 3349026..3349400 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E4F32_RS16325 3344554..3347703 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
E4F32_RS16330 3347952..3348649 + 698 Protein_3172 IS1 family transposase -
E4F32_RS16335 3349026..3349400 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
E4F32_RS16340 3349426..3349644 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
E4F32_RS16345 3349816..3350367 + 552 WP_000102564.1 maltose O-acetyltransferase -
E4F32_RS16350 3350483..3350953 + 471 WP_000136192.1 YlaC family protein -
E4F32_RS16355 3351146..3351843 + 698 Protein_3177 IS1-like element IS1A family transposase -
E4F32_RS16360 3351894..3353444 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
E4F32_RS16365 3353486..3353839 - 354 Protein_3179 DUF1428 family protein -
E4F32_RS16375 3354218..3354529 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside IScluster/Tn - - 3347952..3351843 3891


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T120765 WP_001291435.1 NZ_CP038180:3349426-3349644 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T120765 NZ_CP038180:3349426-3349644 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTTTGGAAATTTATTCGCTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT120765 WP_000344800.1 NZ_CP038180:3349026-3349400 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT120765 NZ_CP038180:3349026-3349400 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References