Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1062839..1063410 Replicon chromosome
Accession NZ_CP038177
Organism Enterococcus faecium strain HN11

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag E3O58_RS05660 Protein ID WP_002302307.1
Coordinates 1062839..1063180 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag E3O58_RS05665 Protein ID WP_002323011.1
Coordinates 1063180..1063410 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3O58_RS05635 (E3O58_05855) 1058223..1058897 - 675 WP_002291233.1 response regulator transcription factor -
E3O58_RS05640 (E3O58_05860) 1059080..1059973 - 894 WP_002326335.1 class C sortase -
E3O58_RS05645 (E3O58_05865) 1060047..1060289 - 243 Protein_1023 LPXTG cell wall anchor domain-containing protein -
E3O58_RS05650 (E3O58_05870) 1060367..1061526 + 1160 WP_265460886.1 IS3 family transposase -
E3O58_RS05655 (E3O58_05880) 1061805..1061990 - 186 WP_002304207.1 hypothetical protein -
E3O58_RS05660 (E3O58_05885) 1062839..1063180 - 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E3O58_RS05665 (E3O58_05890) 1063180..1063410 - 231 WP_002323011.1 hypothetical protein Antitoxin
E3O58_RS05670 (E3O58_05900) 1063698..1064036 + 339 WP_002286804.1 hypothetical protein -
E3O58_RS05675 (E3O58_05905) 1064241..1064816 - 576 WP_010718317.1 SOS response-associated peptidase family protein -
E3O58_RS05680 (E3O58_05910) 1065181..1065762 - 582 WP_043902871.1 TetR/AcrR family transcriptional regulator -
E3O58_RS05685 (E3O58_05915) 1065945..1066571 - 627 WP_002286816.1 cysteine hydrolase -
E3O58_RS05690 (E3O58_05920) 1066593..1067921 - 1329 WP_010718318.1 FAD-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T120747 WP_002302307.1 NZ_CP038177:c1063180-1062839 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T120747 NZ_CP038177:c1063180-1062839 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT120747 WP_002323011.1 NZ_CP038177:c1063410-1063180 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT120747 NZ_CP038177:c1063410-1063180 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References