120746

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) omega-epsilon-zeta/zeta-epsilon
Location 10622..11759 Replicon plasmid pHN11
Accession NZ_CP038176
Organism Enterococcus faecium strain HN11

Toxin (Protein)


Gene name zeta Uniprot ID Q54944
Locus tag E3O58_RS00085 Protein ID WP_001284311.1
Coordinates 10622..11485 (-) Length 288 a.a.

Antitoxin (Protein)


Gene name epsilon Uniprot ID R2XCR7
Locus tag E3O58_RS00090 Protein ID WP_000301765.1
Coordinates 11487..11759 (-) Length 91 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3O58_RS00055 (E3O58_00050) 6460..6687 + 228 Protein_10 hypothetical protein -
E3O58_RS00060 (E3O58_00055) 6711..7619 - 909 WP_002324522.1 IS982-like element ISEfm1 family transposase -
E3O58_RS00065 (E3O58_00060) 7738..7989 + 252 Protein_12 GNAT family N-acetyltransferase -
E3O58_RS00070 (E3O58_00065) 8082..8876 + 795 WP_001096887.1 aminoglycoside O-phosphotransferase APH(3')-IIIa -
E3O58_RS00075 (E3O58_00075) 9414..9497 + 84 WP_128704354.1 23S rRNA methyltransferase attenuation leader peptide -
E3O58_RS00080 (E3O58_00080) 9616..10359 + 744 Protein_15 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(B) -
E3O58_RS00085 (E3O58_00085) 10622..11485 - 864 WP_001284311.1 zeta toxin family protein Toxin
E3O58_RS00090 (E3O58_00090) 11487..11759 - 273 WP_000301765.1 antitoxin Antitoxin
E3O58_RS00095 (E3O58_00095) 11776..11991 - 216 WP_001835296.1 peptide-binding protein -
E3O58_RS00100 (E3O58_00100) 12083..12415 - 333 Protein_19 chromosome partitioning protein ParA -
E3O58_RS00105 (E3O58_00105) 12470..13150 - 681 WP_001015311.1 IS6-like element IS1216 family transposase -
E3O58_RS00110 (E3O58_00110) 13359..13571 + 213 WP_002349227.1 hypothetical protein -
E3O58_RS00115 (E3O58_00115) 13733..14251 + 519 WP_000357244.1 YfbU family protein -
E3O58_RS00120 (E3O58_00120) 14258..14770 + 513 WP_000774078.1 hypothetical protein -
E3O58_RS00125 (E3O58_00125) 15041..15694 + 654 WP_002347609.1 recombinase family protein -
E3O58_RS00130 (E3O58_00130) 15732..16412 + 681 WP_001015311.1 IS6-like element IS1216 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Mobilizable plasmid ant(6)-Ia / aph(3')-III / erm(B) / dfrG / tet(L) / tet(M) / poxtA / fexB - 1..69757 69757
- inside IScluster/Tn ant(6)-Ia / aph(3')-III / erm(B) - 151..16412 16261


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Zeta toxin domain

(27-214)

Antitoxin

Bacterial epsilon antitoxin

(2-90)


Sequences

Toxin        


Download         Length: 288 a.a.        Molecular weight: 32404.02 Da        Isoelectric Point: 6.9964

>T120746 WP_001284311.1 NZ_CP038176:c11485-10622 [Enterococcus faecium]
MANIVNFTDKQFENRLNDNLEELIQGKKAVESPTAFLLGGQPGSGKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELV
KLYEKDVVKHVTPYSNRMTEAIISRLSDQGYNLVIEGTGRTTDVPIQTATMLQAKGYETKMYVMAVPKINSYLGTIERYE
TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETPSISPKETLEKELNRKVSGKEIQ
PTLERIEQKMVLNKHQETPEFKAIQQKLESLQPPTPPIPKTPKLPGI

Download         Length: 864 bp

>T120746 NZ_CP038176:c11485-10622 [Enterococcus faecium]
ATGGCAAATATAGTCAATTTTACTGACAAACAATTTGAGAATCGCTTAAATGATAATTTAGAAGAATTGATTCAAGGAAA
AAAAGCGGTTGAATCGCCAACCGCTTTTTTACTTGGTGGGCAACCAGGGTCAGGGAAAACCAGTTTGCGATCAGCAATTT
TTGAAGAAACACAAGGGAATGTTATTGTCATTGATAATGATACCTTTAAACAACAGCACCCTAATTTTGATGAACTAGTG
AAACTTTATGAAAAAGACGTAGTAAAACACGTTACCCCTTATTCTAATCGCATGACAGAAGCGATCATAAGCCGTTTGAG
CGATCAAGGGTATAATTTGGTGATCGAAGGTACAGGACGAACAACAGACGTTCCTATTCAAACCGCAACAATGCTTCAAG
CCAAAGGTTATGAAACAAAAATGTATGTCATGGCAGTACCTAAAATCAACTCATATTTAGGAACAATTGAACGATATGAA
ACCATGTATGCAGATGATCCAATGACAGCCAGGGCAACACCAAAACAAGCGCATGATATTGTTGTCAAAAACTTACCGAC
CAATTTAGAAACCCTTCATAAAACGGGCTTATTTAGCGATATAAGGCTATATAACAGAGAAGGAGTAAAACTCTATTCAA
GTTTGGAAACACCTTCTATTAGTCCAAAAGAAACCTTAGAAAAAGAATTGAATCGTAAAGTATCAGGGAAAGAAATTCAA
CCGACTTTAGAACGAATAGAGCAAAAAATGGTTCTAAATAAACACCAAGAGACACCTGAATTTAAAGCAATTCAACAAAA
ATTGGAAAGCTTGCAGCCACCTACACCACCAATACCCAAAACACCTAAACTTCCAGGTATTTAA

Antitoxin


Download         Length: 91 a.a.        Molecular weight: 10721.25 Da        Isoelectric Point: 6.4676

>AT120746 WP_000301765.1 NZ_CP038176:c11759-11487 [Enterococcus faecium]
MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELNKNDSQLLEVNLLNQLKLAKRVNLFDYSLEELQAVHEYWRSMNRYS
KQVLNKEKVA

Download         Length: 273 bp

>AT120746 NZ_CP038176:c11759-11487 [Enterococcus faecium]
ATGGCAGTTACGTATGAAAAAACATTTGAAATAGAGATCATTAACGAATTATCGGCAAGCGTTTATAATCGAGTATTAAA
CTATGTTTTGAACCATGAATTAAATAAAAATGACTCTCAATTATTGGAAGTCAATTTATTAAACCAATTAAAGCTTGCAA
AACGTGTAAATCTTTTTGATTATTCTTTAGAAGAATTACAAGCCGTTCATGAGTATTGGCGGTCAATGAATCGTTACTCA
AAACAAGTTTTGAATAAAGAGAAAGTGGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 1GVN
PDB 3Q8X

Antitoxin

Source ID Structure
AlphaFold DB A0A829F0A3

References