Detailed information of TA system TA120745

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Xre-MNT/HTH_26(antitoxin)
• Location: 3699..4807
• Replicon: plasmid pHN11
• Accession: NZ_CP038176
• Organism: Enterococcus faecium strain HN11

Toxin (Protein)

• Gene name: MNTss
• Uniprot ID: A0A2H4HI44
• Locus tag: E3O58_RS00040
• Protein ID: WP_029170889.1
• Coordinates: 3938..4807 (+)
• Length: 290 a.a.

Antitoxin (Protein)

• Gene name: Xress
• Uniprot ID: -
• Locus tag: E3O58_RS00035
• Protein ID: WP_159373724.1
• Coordinates: 3699..3923 (+)
• Length: 75 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3O58_RS00010 (E3O58_00005)	157..837	+	681	WP_013646119.1	IS6-like element IS1216 family transposase	-
E3O58_RS00015 (E3O58_00010)	1078..1245	+	168	WP_000898188.1	hypothetical protein	-
E3O58_RS00020 (E3O58_00015)	1249..1575	+	327	WP_000392725.1	hypothetical protein	-
E3O58_RS00025 (E3O58_00020)	1576..1992	+	417	WP_000323438.1	recombinase	-
E3O58_RS00030 (E3O58_00025)	1994..3556	+	1563	WP_000136908.1	recombinase family protein	-
E3O58_RS00035 (E3O58_00030)	3699..3923	+	225	WP_159373724.1	helix-turn-helix transcriptional regulator	Antitoxin
E3O58_RS00040 (E3O58_00035)	3938..4807	+	870	WP_029170889.1	nucleotidyltransferase domain-containing protein	Toxin
E3O58_RS00045 (E3O58_00040)	4788..5522	+	735	WP_000662263.1	class I SAM-dependent methyltransferase	-
E3O58_RS00050 (E3O58_00045)	5555..6463	+	909	WP_001255866.1	aminoglycoside nucleotidyltransferase ANT(6)-Ia	-
E3O58_RS00055 (E3O58_00050)	6460..6687	+	228	Protein_10	hypothetical protein	-
E3O58_RS00060 (E3O58_00055)	6711..7619	-	909	WP_002324522.1	IS982-like element ISEfm1 family transposase	-
E3O58_RS00065 (E3O58_00060)	7738..7989	+	252	Protein_12	GNAT family N-acetyltransferase	-
E3O58_RS00070 (E3O58_00065)	8082..8876	+	795	WP_001096887.1	aminoglycoside O-phosphotransferase APH(3')-IIIa	-
E3O58_RS00075 (E3O58_00075)	9414..9497	+	84	WP_128704354.1	23S rRNA methyltransferase attenuation leader peptide	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	ant(6)-Ia / aph(3')-III / erm(B) / dfrG / tet(L) / tet(M) / poxtA / fexB	-	1..69757	69757
	-	inside	IScluster/Tn	ant(6)-Ia / aph(3')-III / erm(B)	-	151..16412	16261

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polymerase, nucleotidyl transferase domain (12-114)

Antitoxin

• Cro/C1-type helix-turn-helix domain (4-57)

Sequences

Toxin

Protein

• Length: 290 a.a.
• Molecular weight: 32820.25 Da
• Isoelectric Point: 4.9564

>T120745 WP_029170889.1 NZ_CP038176:3938-4807 [Enterococcus faecium]
MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIYYNSDSFDLTAINQIATELDDKNRNNLVVPPGAWGDW
INGGGWLFINGYHVDLILRDIKRVEQIMKDTEHGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAETY
PNALQKSLVNFFMFEAGFSLMFVKANSGTDDKYYIAGHVFRIVSCLNQVLFACNNAYCINEKKAIKLLETFEHKPEKYTE
KVNHIFEVLGISLFECYDMTEKLYNEVNEIVSEINNFLNEESSDERKQI

Nucleotide

• Length: 870 bp

>T120745 NZ_CP038176:3938-4807 [Enterococcus faecium]
ATGGTAGGTAATGTTATTCAAATAGTAACAGAGAAATTATCATCTTTGCCTTTTATAGAAGGCATCGTATTAGGGGGCTC
ACGTGCAAGAGGCACCCATACAGAAAATTCTGATATAGATATCGGAATCTATTACAATTCAGACTCATTTGACCTGACAG
CGATTAATCAAATTGCTACAGAATTGGATGATAAAAATAGAAACAACCTTGTTGTGCCTCCCGGAGCGTGGGGTGATTGG
ATTAATGGCGGCGGATGGTTATTTATAAATGGGTATCATGTGGATTTAATTTTACGTGATATTAAACGTGTGGAACAAAT
AATGAAAGATACAGAGCACGGAATTGTTACTGCCAATTATCAGACTGGGCATCCCCATGGTTATATTAGTGCAATGTATC
GAGGAGAATTAGCGATTAGCAAAATACTATATGCTAAGAATGAAAGCTTATGCGAATTAAAAAAACAGGCAGAAACTTAT
CCCAATGCTTTGCAGAAAAGTTTAGTTAACTTTTTTATGTTTGAAGCAGGGTTCTCTTTAATGTTTGTAAAAGCAAATTC
GGGAACAGACGATAAATATTATATTGCGGGTCATGTTTTTCGTATAGTTTCATGTTTAAATCAAGTGTTATTTGCATGTA
ATAATGCTTATTGTATCAACGAAAAGAAAGCTATAAAACTGCTTGAAACTTTTGAACATAAACCTGAAAAATATACCGAG
AAGGTAAATCATATTTTTGAAGTACTCGGTATCTCACTTTTTGAATGCTACGACATGACCGAGAAGCTTTATAATGAAGT
GAATGAAATTGTATCGGAGATAAATAACTTTTTAAACGAGGAGAGTTCAGATGAAAGAAAACAAATATGA

Antitoxin

Protein

• Length: 75 a.a.
• Molecular weight: 8347.65 Da
• Isoelectric Point: 4.5510

>AT120745 WP_159373724.1 NZ_CP038176:3699-3923 [Enterococcus faecium]
MTTAEIIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKFVQAFILPDGEEIKISNE

Nucleotide

• Length: 225 bp

>AT120745 NZ_CP038176:3699-3923 [Enterococcus faecium]
ATGACTACGGCAGAAATAATTAAAGAACTGTGTGAGCAAATGAATATAAGTGTTTCCGAACTTGCTAGACGTATTGGCCA
GACTCCACAGAATTTCAATAAAAAATTACAACGAGAAACAGTAACCTTGGATGAGTTGAAGGCCATTGCTGATGTGCTAG
GTGTCAAGTTTGTGCAGGCATTTATTTTACCAGATGGAGAAGAAATAAAAATATCTAACGAATAA

Structures

Toxin

• AlphaFold DB: A0A2H4HI44