120742

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 1449334..1449905 Replicon chromosome
Accession NZ_CP038174
Organism Enterococcus faecium strain SDGJQ5

Toxin (Protein)


Gene name mazF Uniprot ID Q3Y2B8
Locus tag E3T52_RS07155 Protein ID WP_002286801.1
Coordinates 1449334..1449675 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag E3T52_RS07160 Protein ID WP_002323011.1
Coordinates 1449675..1449905 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3T52_RS07130 (E3T52_07435) 1444545..1445879 - 1335 WP_283651788.1 ABC transporter substrate-binding protein -
E3T52_RS07135 (E3T52_07440) 1446087..1446917 + 831 WP_002286789.1 manganese catalase family protein -
E3T52_RS07140 (E3T52_07450) 1447161..1447410 - 250 Protein_1395 transposase -
E3T52_RS07145 (E3T52_07455) 1447601..1447795 - 195 WP_002295146.1 hypothetical protein -
E3T52_RS07150 (E3T52_07460) 1448300..1448485 - 186 WP_002304207.1 hypothetical protein -
E3T52_RS07155 (E3T52_07465) 1449334..1449675 - 342 WP_002286801.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E3T52_RS07160 (E3T52_07470) 1449675..1449905 - 231 WP_002323011.1 hypothetical protein Antitoxin
E3T52_RS07165 (E3T52_07480) 1450192..1450530 + 339 WP_002286804.1 hypothetical protein -
E3T52_RS07170 (E3T52_07485) 1450735..1451310 - 576 WP_002293673.1 SOS response-associated peptidase family protein -
E3T52_RS07175 (E3T52_07500) 1451919..1452500 - 582 WP_002286813.1 TetR/AcrR family transcriptional regulator -
E3T52_RS07180 (E3T52_07505) 1452683..1453309 - 627 WP_002333420.1 cysteine hydrolase -
E3T52_RS07185 (E3T52_07510) 1453331..1454662 - 1332 WP_002286818.1 FAD-containing oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13302.68 Da        Isoelectric Point: 9.9044

>T120742 WP_002286801.1 NZ_CP038174:c1449675-1449334 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T120742 NZ_CP038174:c1449675-1449334 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTACACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT120742 WP_002323011.1 NZ_CP038174:c1449905-1449675 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT120742 NZ_CP038174:c1449905-1449675 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829FD66

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References