Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/PRK09907(toxin)
Location 243886..244457 Replicon chromosome
Accession NZ_CP038172
Organism Enterococcus faecium strain YN2-1

Toxin (Protein)


Gene name mazF Uniprot ID A0A828ZXV4
Locus tag E3T57_RS01250 Protein ID WP_002302307.1
Coordinates 243886..244227 (-) Length 114 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID A0A828ZZN9
Locus tag E3T57_RS01255 Protein ID WP_002323011.1
Coordinates 244227..244457 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
E3T57_RS01225 (E3T57_01305) 239271..239945 - 675 WP_002291233.1 response regulator transcription factor -
E3T57_RS01230 (E3T57_01310) 240128..241021 - 894 WP_002326335.1 class C sortase -
E3T57_RS01235 (E3T57_01315) 241095..241337 - 243 Protein_245 LPXTG cell wall anchor domain-containing protein -
E3T57_RS01240 (E3T57_01320) 241415..242573 + 1159 Protein_246 IS3-like element IS1485 family transposase -
E3T57_RS01245 (E3T57_01330) 242852..243037 - 186 WP_002304207.1 hypothetical protein -
E3T57_RS01250 (E3T57_01335) 243886..244227 - 342 WP_002302307.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
E3T57_RS01255 (E3T57_01340) 244227..244457 - 231 WP_002323011.1 hypothetical protein Antitoxin
E3T57_RS01260 (E3T57_01350) 244745..245083 + 339 WP_002286804.1 hypothetical protein -
E3T57_RS01265 (E3T57_01355) 245288..245863 - 576 WP_010718317.1 SOS response-associated peptidase family protein -
E3T57_RS01270 (E3T57_01360) 246228..246809 - 582 WP_043902871.1 TetR/AcrR family transcriptional regulator -
E3T57_RS01275 (E3T57_01365) 246992..247618 - 627 WP_002286816.1 cysteine hydrolase -
E3T57_RS01280 (E3T57_01370) 247640..248968 - 1329 WP_010718318.1 FAD-dependent oxidoreductase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- flank IS/Tn - - 241415..241705 290


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(10-113)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13288.65 Da        Isoelectric Point: 9.9044

>T120740 WP_002302307.1 NZ_CP038172:c244227-243886 [Enterococcus faecium]
VSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Download         Length: 342 bp

>T120740 NZ_CP038172:c244227-243886 [Enterococcus faecium]
GTGTCAGAAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTGATTTTGATCCCTCCGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8523.39 Da        Isoelectric Point: 3.9078

>AT120740 WP_002323011.1 NZ_CP038172:c244457-244227 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Download         Length: 231 bp

>AT120740 NZ_CP038172:c244457-244227 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGATGATGGAACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A828ZXV4

Antitoxin

Source ID Structure
AlphaFold DB A0A828ZZN9

References