Detailed information of TA system TA120739

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2390259..2390830
• Replicon: chromosome
• Accession: NZ_CP038170
• Organism: Enterococcus faecium strain SDGJP3

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3T45_RS11850
• Protein ID: WP_002328559.1
• Coordinates: 2390489..2390830 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: E3T45_RS11845
• Protein ID: WP_002323011.1
• Coordinates: 2390259..2390489 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T45_RS11820 (E3T45_12460)	2385377..2386705	+	1329	WP_002327663.1	FAD-containing oxidoreductase	-
E3T45_RS11825 (E3T45_12465)	2386727..2387353	+	627	WP_002286816.1	cysteine hydrolase	-
E3T45_RS11830 (E3T45_12470)	2387536..2388117	+	582	WP_002286813.1	TetR/AcrR family transcriptional regulator	-
E3T45_RS11835 (E3T45_12490)	2388849..2389424	+	576	WP_002327664.1	SOS response-associated peptidase family protein	-
E3T45_RS11840 (E3T45_12495)	2389625..2389963	-	339	WP_002327665.1	hypothetical protein	-
E3T45_RS11845 (E3T45_12505)	2390259..2390489	+	231	WP_002323011.1	hypothetical protein	Antitoxin
E3T45_RS11850 (E3T45_12510)	2390489..2390830	+	342	WP_002328559.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3T45_RS11855 (E3T45_12515)	2391649..2392811	+	1163	WP_143351449.1	IS3 family transposase	-
E3T45_RS11860 (E3T45_12525)	2392942..2394126	+	1185	Protein_2279	SpaA isopeptide-forming pilin-related protein	-
E3T45_RS11865 (E3T45_12530)	2394126..2395439	+	1314	WP_002328556.1	pilin N-terminal domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13278.70 Da
• Isoelectric Point: 10.1461

>T120739 WP_002328559.1 NZ_CP038170:2390489-2390830 [Enterococcus faecium]
VSGERIYIPKKGDIVWIYFDPSLGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T120739 NZ_CP038170:2390489-2390830 [Enterococcus faecium]
GTGTCAGGAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTTATTTTGATCCCTCATTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT120739 WP_002323011.1 NZ_CP038170:2390259-2390489 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT120739 NZ_CP038170:2390259-2390489 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGACGATGGTACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A828ZZN9