Detailed information of TA system TA120737

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 2136175..2136746
• Replicon: chromosome
• Accession: NZ_CP038166
• Organism: Enterococcus faecium strain SC3-1

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3O61_RS10390
• Protein ID: WP_002327666.1
• Coordinates: 2136405..2136746 (+)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: E3O61_RS10385
• Protein ID: WP_002323011.1
• Coordinates: 2136175..2136405 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3O61_RS10360 (E3O61_10870)	2131577..2132203	+	627	WP_002286816.1	cysteine hydrolase	-
E3O61_RS10365 (E3O61_10875)	2132386..2132967	+	582	WP_002286813.1	TetR/AcrR family transcriptional regulator	-
E3O61_RS10370 (E3O61_10895)	2133699..2134274	+	576	WP_002327664.1	SOS response-associated peptidase family protein	-
E3O61_RS10375 (E3O61_10900)	2134475..2134813	-	339	WP_002327665.1	hypothetical protein	-
E3O61_RS10380 (E3O61_10905)	2134971..2135928	-	958	Protein_1989	IS30-like element IS6770 family transposase	-
E3O61_RS10385 (E3O61_10910)	2136175..2136405	+	231	WP_002323011.1	hypothetical protein	Antitoxin
E3O61_RS10390 (E3O61_10915)	2136405..2136746	+	342	WP_002327666.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3O61_RS10395 (E3O61_10920)	2137610..2138715	-	1106	Protein_1992	IS3 family transposase	-
E3O61_RS10400 (E3O61_10925)	2139192..2139377	-	186	WP_002304207.1	hypothetical protein	-
E3O61_RS10405 (E3O61_10930)	2139617..2140725	+	1109	Protein_1994	IS3 family transposase	-
E3O61_RS10410 (E3O61_10935)	2141008..2141202	+	195	WP_002295146.1	hypothetical protein	-
E3O61_RS10415 (E3O61_10940)	2141393..2141642	+	250	Protein_1996	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ebpC	2054001..2266504	212503
	-	inside	IScluster/Tn	-	-	2134971..2140725	5754

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13264.67 Da
• Isoelectric Point: 10.1461

>T120737 WP_002327666.1 NZ_CP038166:2136405-2136746 [Enterococcus faecium]
VSGERIYIPKKGDIVWIYFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T120737 NZ_CP038166:2136405-2136746 [Enterococcus faecium]
GTGTCAGGAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTTATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT120737 WP_002323011.1 NZ_CP038166:2136175-2136405 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT120737 NZ_CP038166:2136175-2136405 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGACGATGGTACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A828ZZN9