Detailed information of TA system TA120734

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PRK09907(toxin)
• Location: 1133419..1133990
• Replicon: chromosome
• Accession: NZ_CP038164
• Organism: Enterococcus faecium strain HB2-2

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: E3O52_RS05425
• Protein ID: WP_002327666.1
• Coordinates: 1133419..1133760 (-)
• Length: 114 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: A0A828ZZN9
• Locus tag: E3O52_RS05430
• Protein ID: WP_002323011.1
• Coordinates: 1133760..1133990 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3O52_RS05410 (E3O52_05625)	1129549..1130721	-	1173	WP_146708620.1	IS256-like element IS256 family transposase	-
E3O52_RS05415	1130782..1130967	+	186	WP_272025088.1	hypothetical protein	-
E3O52_RS05420 (E3O52_05635)	1131444..1132554	+	1111	Protein_1072	IS3-like element IS1485 family transposase	-
E3O52_RS05425 (E3O52_05640)	1133419..1133760	-	342	WP_002327666.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
E3O52_RS05430 (E3O52_05645)	1133760..1133990	-	231	WP_002323011.1	hypothetical protein	Antitoxin
E3O52_RS05435 (E3O52_05655)	1134286..1134624	+	339	WP_002327665.1	hypothetical protein	-
E3O52_RS05440 (E3O52_05660)	1134825..1135400	-	576	WP_002327664.1	SOS response-associated peptidase family protein	-
E3O52_RS05445 (E3O52_05680)	1136075..1136656	-	582	WP_002286813.1	TetR/AcrR family transcriptional regulator	-
E3O52_RS05450 (E3O52_05685)	1136839..1137465	-	627	WP_002286816.1	cysteine hydrolase	-
E3O52_RS05455 (E3O52_05690)	1137487..1138815	-	1329	WP_002327663.1	FAD-containing oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	ebpC	1024744..1192417	167673

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-112)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 114 a.a.
• Molecular weight: 13264.67 Da
• Isoelectric Point: 10.1461

>T120734 WP_002327666.1 NZ_CP038164:c1133760-1133419 [Enterococcus faecium]
VSGERIYIPKKGDIVWIYFDPSVGKEIQKRRPGLVVSRYEFNRKTMFSVICPITSTIKNLPTRYSLPEELDIKGQVIISQ
LKSLDFKERKLKKIENLPLQDMAKIDQIIQYMF

Nucleotide

• Length: 342 bp

>T120734 NZ_CP038164:c1133760-1133419 [Enterococcus faecium]
GTGTCAGGAGAAAGAATTTATATTCCTAAAAAAGGTGATATTGTATGGATTTATTTTGATCCCTCAGTAGGAAAAGAAAT
TCAAAAAAGACGACCAGGACTAGTCGTCTCTCGTTATGAATTTAACCGTAAAACAATGTTTTCGGTCATATGTCCAATTA
CTTCCACTATAAAGAATTTACCGACACGTTACTCATTACCTGAAGAGCTAGATATAAAAGGACAAGTAATTATTTCACAA
TTAAAATCTCTTGATTTTAAGGAACGAAAACTCAAAAAAATAGAAAACTTACCATTGCAAGACATGGCTAAAATTGATCA
GATTATACAGTATATGTTTTAA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8523.39 Da
• Isoelectric Point: 3.9078

>AT120734 WP_002323011.1 NZ_CP038164:c1133990-1133760 [Enterococcus faecium]
MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLVPKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL

Nucleotide

• Length: 231 bp

>AT120734 NZ_CP038164:c1133990-1133760 [Enterococcus faecium]
ATGCTTGTTACAAAATCTAGATTACAAGGAAGTTCTGTAGTGGTAACTTTACCTTCTGATAACGGAAAGAAACCTTCAGA
AAACCAAGAATATATCGTTGTGTATTCTGACGATGGTACAATTACGTTAGTTCCAAAAATTGAAGATCCATTTAGTGGTG
GGGAAGAGGCCGAGTATTATGAGAAAGACGAATGGGAAGATTTAACTCCTGAAGGGAGAGAAATTTTATAG

Structures

Antitoxin

• AlphaFold DB: A0A828ZZN9